...should be easy, right? Yes and no. If you’re just trying to create a knockout, you might be happy targeting...

...tagging does have its limitations, especially when trying to perform high-throughput experiments. If a user...

... of CRISPR samples. We invite you to jump in and try it out, or take a look at our live examples: experiment...

...cell. Typically when people use plasmids, they are trying to do one thing at a time. What the BioBricks Foundation...our dreams and intentions) and the genetics and chemistry of life, then this new language needs to be free...

..., or metastasize. Many cancerous cells will die trying, as the circulatory and lymph systems are not ideal..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...

...NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself On the other hand, the widespread...

... notable exception If you have a sample you are trying to compensate in the top right corner of a flow...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...buttons. For anyone just starting to use flow cytometry, it’s generally recommended to start compensating... vs. Spectral Flow Cytometry...

...Addgene’s repository. Other researchers, eager to try this new technology, were quick to request CRISPR...deposits and distribution by country. (A) Total CRISPR plasmids deposited by country; square size is proportional...requested by country; square size is proportional to the number of plasmids requested by each country. Countries...therefore looked at depositors and requestors by country, and graphed the top ten countries for each group...proportional to number of plasmids deposited by each country. Countries shown are top 10 depositing countries...

...routinely, and it can be especially challenging when trying to develop antibodies against unstable targets ...

...enables a broader targeting scope for base editing.  Try ABE8 base editors for your research! Base editing...

...and informal communications we made a decision to try out a Bioinformatics/HTS a journal club. Sorting ...

...smart dropdown menus below the search bar. As you try different terms you may find the available categories...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists...

...discussing a figure, jot down the question you were trying to answer, the method you used, the main result...

...cells Don’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most...Khilji recently acquired her Master’s degree in Biochemistry from Freie Universität Berlin, Germany. She ...

...opportunity for your classroom, I highly recommend you try implementing one. It's likely only a matter of time... in their careers? A CURE for Biochemistry Undergraduate biochemistry courses are often associated with...Minnesota State (and now Professor of Chemistry and Biochemistry at the University of San Diego), decided...Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth...presented their findings at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of...inquiry and research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification...dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323...

...the scales in your favor for an HDR edit, you can try one or more of the below suggestions. Disable the...

...issues related to AAV vectors, many from people trying to answer the exact same question I was: What AAV...

...strategy for increasing protein production. If you are trying to express a protein in its native host there is...

...applying for technical support positions, you should try to match your expertise with that of the company....are often found in sales or lab-based roles in industry. What to look forward to in a technical support...offer an easy transition from an academic lab to industry without being too commercial or “sales-y”. The...be a good jumping off point for other roles in industry. Many thanks to Eric Perkins and Stephanie Hays...

...organizations like Science from Scientists, which try to bring real scientists into schools to provide ... job” could entail any number of career paths--industry, nonprofit organization, law office, journal editor...