...individual members, many opinions may exist. I learnt to try and bring a group of people to an overall consensus...

...to get your point across. Some supervisors might try to intimidate you with negative comments (hostile-aggressives...

... notable exception If you have a sample you are trying to compensate in the top right corner of a flow...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...In flow cytometry, compensation is the process of correcting spillover from one fluorescent channel to...buttons. For anyone just starting to use flow cytometry, it’s generally recommended to start compensating... vs. Spectral Flow Cytometry...

...Addgene’s repository. Other researchers, eager to try this new technology, were quick to request CRISPR...deposits and distribution by country. (A) Total CRISPR plasmids deposited by country; square size is proportional...requested by country; square size is proportional to the number of plasmids requested by each country. Countries...therefore looked at depositors and requestors by country, and graphed the top ten countries for each group...proportional to number of plasmids deposited by each country. Countries shown are top 10 depositing countries...

...should be easy, right? Yes and no. If you’re just trying to create a knockout, you might be happy targeting...

... of CRISPR samples. We invite you to jump in and try it out, or take a look at our live examples: experiment...

...and informal communications we made a decision to try out a Bioinformatics/HTS a journal club. Sorting ...

...smart dropdown menus below the search bar. As you try different terms you may find the available categories...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists...

...discussing a figure, jot down the question you were trying to answer, the method you used, the main result...

...cells Don’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most...Khilji recently acquired her Master’s degree in Biochemistry from Freie Universität Berlin, Germany. She ...

...routinely, and it can be especially challenging when trying to develop antibodies against unstable targets ...

...enables a broader targeting scope for base editing.  Try ABE8 base editors for your research! Base editing...

...tagging does have its limitations, especially when trying to perform high-throughput experiments. If a user...

..., or metastasize. Many cancerous cells will die trying, as the circulatory and lymph systems are not ideal..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...

...NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself On the other hand, the widespread...

...cell. Typically when people use plasmids, they are trying to do one thing at a time. What the BioBricks Foundation...our dreams and intentions) and the genetics and chemistry of life, then this new language needs to be free...

...location of the protein of interest. You may need to try a variety of lysis buffers to find the best for your...Collection Antibody Blog Posts Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification...

... dictated by the specific type of virus you are trying to make. For example, bacteriophages will need ...

...it was promising enough to keep exploring and to try developing a variant with an enhanced response. Evolution...researchers from a range of fields, like quantum chemistry and protein biophysics, as well as inventors and...effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447...

...factors may impact the dynamic range of the assay. Try to minimize these.  Review previous literature or...