We narrowed to 301 results for: pET

1. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   ...References Loeff, L., Brouns, S. J., & Joo, C. (2018). Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide...

2. Common Injection Routes in Mice

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   Blog Post

   ... is easier, less invasive, and only requires a pipette. Since the substances are administered through ...

3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   .... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...

4. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...blogs, or in the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...that inhibit BiFC in your cellular system. BiFC competition analysis can be used in lieu of negative controls...

6. DNA Purification Without a Kit

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   Blog Post

   ...mammalian cells in 30 seconds or less without using a pipette.  Here’s how this method works: nucleic acids are...

7. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...

8. Summer SciComm Series: Modes of Communication

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   Blog Post

   ... needed. Traditional media formats are quite competitive while digital can be oversaturated. Transition...

9. MXS Chaining

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   Blog Post

   ...for the modular assembly of highly similar or repetitive sequences. Intermediate chaining products (called...

10. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...

11. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

12. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard flow-through solutions...

13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ...been full of dark matter: enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We...

14. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    .... "Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics...

15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...survive as a researcher because of the tough competition for academic positions and poor grant situation...

16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ...on OWW, including those for preparing your own competent cells. If you’re familiar with the process of ...

17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post

    ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered...

18. Hot Plasmids - August 2020

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    Blog Post

    ...plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate...

19. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ...robust research proposal will distinguish between competing models. Comparing model A vs. B is more constructive...

20. Lab to Office Culture Shock

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    Blog Post

    ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...