Antibodies 101: Choosing the Right Isotype
Type
Blog Post
...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice.
Target and application should be considered
The first question you may... a strong signal and to work in a variety of applications.
The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed...