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  1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...two genes are found between the AAV genome’s two inverted terminal repeats (ITRs); but when recombinant ...through a pore in the capsid. Rep proteins are also involved in the transcriptional regulation of the AAV p40...

  3. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...discovery takes one of two approaches. The first route involves identifying a gene/pathway to develop a drug for...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ...

  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are called...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... famous role as the “happy hormone,” it’s also involved in reinforcement learning, decision making, and...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...

  8. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...Remember that people like a good story and science involves characters – in this case you – on a quest to ... and hopefully discover something new. Science involves success and, more often, failure. It’s useful ...

  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...determine the binding kinetics of a purified protein involves immobilization on a solid support. This allows...selectivity A common strategy to biotinylate proteins involves amine-reactive biotin derivatives that generate...

  10. The Strength of Story Telling

    Type
    Blog Post
    ...identifying with a character, building emotional investment and following cause-and-effect relationships ...address far and wide, keep your website up to date, invite people to ask questions, be approachable. Engage...

  11. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...be a frustrating process that requires a large investment of time and resources for labs that don’t have...are used in experiments other than just those involving CRISPR and so we also have lentivirus for delivering...

  12. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized Argonaute...hypothesized that base removal helps prevent the invading genome from “recovering” its original sequence...

  13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Do you think there are topics we’re missing? We invite you to suggest topics you’d like to see on the ...Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It also provides...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...science communication. The first year of the course involved a whirlwind of skill acquisition. Classes covered...utilize a wide array of media in doing so—can prove invaluable in the pursuit of public engagement. Studying...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus ...., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation...

  17. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...
Showing: 201 - 220 of 525 results