.... Other dyes may prove challenging to stain intracellular compartments in in vivo model systems, so extra...green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(Hayashi-Takanaka...fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces-2 contains...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the...

...barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always be able...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...of support and structure, prevent science from attracting and keeping diverse talent in science related...prioritize ongoing work.  If we’re going to continue attracting and launching science talent we must ensure that...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping...

...Blog Learn How the Clon Tracer Library Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be ...

...Queensland, whose outstanding marine photography has attracted much attention over the past few years, including...is easy to see how these incredible images are attracting so much attention. When I spoke with Daniel about...

...part of Hans’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have...communication with the presenters, and editing of abstracts and speaker biographies.” During the week of ...

...plasmids to self-replicate within a cell make them attractive tools for the life scientist or bioengineer. ...Produce proteins that glow so that scientists can track their location or quantity inside a cell Plasmids...

...members. We also find that, as a nonprofit, we attract partnerships with organizations that share our ...Renee, Shannon, Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie, and Xuan Additional Resources...

...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

... looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for...your lab protocols using apps to save you time and track your experiments  Resources on Addgene.org: Read...

...deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution tracking of more than...

...mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy of mitochondria, or mitophagy...Rosella can also be fused to a protein of interest to track the autophagy of that protein. Find the cytosol-targeted...

...modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their growth and extension...Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive...

...out of a complex mix of proteins that have been extracted from a cell, instead of when it is attached to...certain conditions? It can help to visualize an abstract protein during your sample prep process, imagining...

...suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction protocol for...

...ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura, a professor at ...