Addgene

We narrowed to 243 results for: TIR

Showing: 201 - 220 of 243 results

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Site Directed Mutagenesis by PCR

Type

Blog Post

... mutation) in a PCR protocol that amplifies the entire plasmid template. The parent template is removed...
Evolution of Lab Techniques

Type

Blog Post

...sequencing technologies could one day sequence an entire genome for under $1,000 (10). The future of sequencing...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

...solutions that could save both your lab and the entire research enterprise time and funds. It’s time to...
Viral Vectors 101: Optogenetic Tools

Type

Blog Post

... photoswitches OptoXRs are great for activating entire signaling pathways, but what if you’re interested...
CRISPR 101: Off-Target Effects

Type

Blog Post

...sequencing The only way to quantify off-target effects entirely is to perform whole genome sequencing. While this...
Plasmids 101: Dimers and Multimers

Type

Blog Post

... on as a dimer, depicted on the right, with the entire monomeric sequence duplicated in tandem, resulting...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

...real-time correspondence via e-mail throughout the entire year. In addition to their direct training role...
RNA Interference in Plant Biology: New Tools for an Old Favorite

Type

Blog Post

...trivializes downstream characterization, as the entire culture can be harvested instead of microscopic...
Transferable Skills Guide: Teamwork

Type

Blog Post

...more things necessary to make a successful team. Entire books have been written about this! But this is...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

...gene editing process. See below for details. The entire process from start to finish took about 1 month...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...2) if the dsDNA was short enough, a ssDNA whose entire complementary strand was degraded. Beta: Beta protects...
A Guide to Getting Started in Undergrad Research

Type

Blog Post

...industry, so feel free to explore! Addgene has an entire page dedicated to different career options from...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Type

Blog Post

...Shuttle device; 5 days after the transfection, the entire plate was scanned for V5 tagging using an Operetta...
A Needle in a Base-Stack: Cas9 Structural Biology

Type

Blog Post

...the recognition domains—including the CTD and the entire REC lobe—do not structurally resemble any other...
How to Deposit Your Plasmids with Addgene

Type

Blog Post

...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...
Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Type

Blog Post

...guide Find CRISPR plasmids by function Browse our entire plasmid collection ...
Antibody Validation for Flow Cytometry

Type

Blog Post

...relative protein expression. Its reliability will be entirely dependent on the selectivity of the antibody used...
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Type

Blog Post

... Advanced functions: These functions are not entirely necessary but should be considered extremely useful...

Showing: 201 - 220 of 243 results

Results per page:

Site Directed Mutagenesis by PCR

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Antibodies 101: Single Chain Fragment Variables (scFvs)

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Viral Vectors 101: Optogenetic Tools

CRISPR 101: Off-Target Effects

Plasmids 101: Dimers and Multimers

Building Global Connections with the International Mentorship Program USA-EUROPE

RNA Interference in Plant Biology: New Tools for an Old Favorite

Transferable Skills Guide: Teamwork

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

A Guide to Getting Started in Undergrad Research

Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

A Needle in a Base-Stack: Cas9 Structural Biology

How to Deposit Your Plasmids with Addgene

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Antibody Validation for Flow Cytometry

The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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