... Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4... and Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts...investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie Chen is a co-founder...and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software Engineer...

...current forming membrane pores) transfection. These techniques are still used and efficiently transfer plasmid...not been tested for HIV.  If not using proper technique, there’s always a small chance that a researcher...transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly after transfection...for virus titration: the p24 ELISA dosing method detects the expression of viral capsid antigen p24, the...Therapy, Dr. Ke Xu (Ed.), ISBN: 978-953-307-539-6, InTech. 4. Walter, W., and U. Stein. "Viral vectors for...

... itself was relatively easy. Figure 4: Detection of edited BRAF genomic loci in Cas9-expressing...dsDNA) at basepair mismatches. To use this tool to detect a BRAF gene edit, simply PCR-amplify the BRAF locus...be in the lab. Based on the power of available techniques and how quick and easy it is to share information...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

...research.     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform...high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or DNA. Now they have developed...developed this further to provide multiplexed detection, higher sensitivity, and more. Read the paper...and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene blog post...

... modular, standardized, disciplined, and open techniques. Chapter 4: Research projects underway in the...Synthetic Biology. Wide adoption of synthetic biology techniques in biology research as can already be seen with...University of Cambridge before spending time in biotech screening direct interactions between drugs and...investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising...

...backgrounds! Show off your favorite reagent, scientific technique, or plasmid repository at your next meeting.   ...blog topic to learn more about FRET and other techniques using FPs! Otherwise, check out our plasmid and... browse plasmids by field, organism, research technique, and more. Download the green DNA background. ...those backgrounds representative of your favorite technique? Our lab-themed background is a great fit for ...

...depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein...reagent sharing even broader and began working with tech transfer offices to make reagents available to scientists...extracting RNA without a kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh...University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids deposited...

...classification system for the array of CRISPR technologies. Learning the general principles of this classification...systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is largely...the first taught when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved...Y. (2018). Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing. Cell ...Yoshimi, K., & Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system...

...this post even more useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins...editor’s choice posts this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins...from the University of Alberta and describes a technique you can use to find phage proteins that bind to...2) The post describes an ingenious but simple technique to screen for proteins that could be useful for...

...are swarming to see what can be done with this technology to help patients. Dr. Stoffels and others in ...months compared to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents..., the panelists agreed that, for example, the technology is already available today to cure sickle cell...really sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History...

...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

...sensitive detection of the plant hormones strigolactones By Fabienne Furt Current methods used to detect strigolactones...CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New ready-to-...intensity, it provides a direct and quantitative detection of SLs. The authors also showed that the receptor-based...

...purification tools (and can have secondary functions as detection aids.) When a single protein is expressed, with...from solution. MBP antibodies are available for detection and/or purification of MBP-tagged proteins. The...alternative to amylose resin and also provides a visual detection mechanism via applications such as western blot...of interest, especially if downstream antibody detection is desired… and yes, we have an antibody for that...

...limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available imaging...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint...each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic collapse... in relation to internal organs. The limit of detection for arg1 expressing ECN is 109 cells/ml, which...

...material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking during...improvement and simplification of the original technique, specifically in terms of initial steps of cell...ChIP-seq, especially in situations when it is technically challenging to collect large numbers of cells...the CUT&RUN method, it seems likely that this technique will quickly become a standard method alongside...

...Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use... linear or circular sequence. Next, we use the “Detect Common Features” function to find and annotate ...s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal...plasmid maps and sequences use the same feature detection software as the desktop version of Snapgene. When...

... be your critical experiments and why? What techniques and skills do you need to use to reach these ...address a topic in a mechanistic way using the technology available.” Choosing a research project “entails...entails good judgement with the current technology to identify a problem, asking what approach will I take... importance of combining “new discoveries or technologies increase chances that your project will be fresh...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ... These questions are focused on injection and detection methods. For example, you can provide location...you wait until performing the experiment? What detection methods did you use for expression of AAV cargo...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources on...

...context of IHC, but the problem can impact other techniques like Western blots or immunocytochemistry (ICC... described here may be as effective for other techniques, but it is important to be aware that the issue...Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free Reagents, Immunofluorescence..., Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding of human...

...no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...called TOPO-TA) cloning; however, the TOPO cloning technique has also be adapted for blunt end cloning. So ... DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase...Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ...

...the chance to win some of the latest tools and technologies in life sciences research. The rare disease ...countries. “Over $600,000 worth of cutting-edge technologies were awarded to study 31 rare diseases,” said...ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit...those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying...

...fluorescent ligands to the loci of interest. Dual color detection In order to label two loci simultaneously, Ma ...CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating MS2 ...target site. Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, ...engineered sgRNAs using CRISPRainbow.” Nature Biotechnology 34 (2016): 528-530. PubMed PMID: 27088723 PubMed...

...Positive vs Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles/...

...experiments in this publication were performed using a technique called nucleofection, which introduces functional...2016, and Kim et al 2014) have shown that this technique supports extremely high frequencies of genome ...experiments to try nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed PMID: 26789497.  2. Lin, Steven...

...analysis blog post. Colony PCR Colony PCR can detect the presence or absence of DNA using lysed bacterial...plasmid. In this case, you’d be using colony PCR to detect your insert, either by using primers that are specific...time-intensive than Sanger sequencing or the other techniques mentioned above. In molecular biology, cloning...By using a variety of screening and selection techniques, you can increase the chances of finding the ...

...revolutionary physical approaches. However, with each new technical achievement, the complexity of the device increases...PhD, I have been convinced that most microscopy techniques can be replicated with commercially available...available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC milling, and... and PCB manufacturing. These newly developed techniques are the key enablers for making assemblies open-source...

...inspire them and also keep up with all the new biotechnology.” These are the types of inquiries that routinely...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...module currently under development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses... DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted in a new BioBuilder ...

...at each locus, as well as large deletions. They detected fragment deletion with 4-20% frequency at the ...you have any questions or thoughts about this technology, leave them in the comments below.  References...plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed and programmable... Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: 10.1038/nbt.2908...

... but this skill is not just about reacting to technical issues. This skill also comes into play while ...encounter. As you spend time in a lab learning techniques and taking on independent projects, you will ...solutions. Ultimately, problem solving is less about technical know-how and more about how you approach new challenges...important. Paired with your other skills, both technical and transferable (i.e. teamwork and time management...

.... We also opened up applications to research technicians and undergraduate researchers, in addition to...Abhi Aggarwal is a postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research...variants of iGluSnFR3 in vivo and have used it to detect glutamate release from hundreds of synapses at ...believe that improved biosensors or biosensors that detect new redox metabolites are vital for researchers...