... purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM MgCl 2 and/or 1µl DMSO to each...

... parents. If both of the mutations that you are trying to cross are located on homologous chromosomes ...

...have many levels of complexity, especially when trying to explore the links between genetics and anatomy...

...role at Addgene is “Words are important!”  If you try any of these approaches in your lab or workplace,...

...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

...autophagy of your protein of interest. Are you ready to try out Rosella? Both the cytosol-targeted and mitochondria-targeted...

... next? First, check the submission deadline and try not to panic if it's due in a few days, weeks, or...

... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...

...individual members, many opinions may exist. I learnt to try and bring a group of people to an overall consensus...

...to get your point across. Some supervisors might try to intimidate you with negative comments (hostile-aggressives...

...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

...efficiency. Before constructing your own FRET probe, try searching PubMed for articles describing the FRET...

...getting sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep...

...location of the protein of interest. You may need to try a variety of lysis buffers to find the best for your...Antibody Plasmid Collection Antibody Blog Posts Immunocytochemistry Protocol Recombinant Antibody Purification...

...pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid or an organism’s ... and Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...

...discussing a figure, jot down the question you were trying to answer, the method you used, the main result...

... of CRISPR samples. We invite you to jump in and try it out, or take a look at our live examples: experiment...

...cells Don’t be lazy about splitting cells, instead try to form a routine. Twice a week often works for most...Khilji recently acquired her Master’s degree in Biochemistry from Freie Universität Berlin, Germany. She ...

...should be easy, right? Yes and no. If you’re just trying to create a knockout, you might be happy targeting...