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We narrowed to 532 results for: invs

Showing: 181 - 200 of 532 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...identifying previously unknown factors and pathways involved in pre-mRNA splicing in a compound screen. ...visualize the fusion in vitro or in vivo. The protocol involves isolating total RNA, resolving by PAGE and then...

  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...Stability of fluorescence: For applications that involve ranges of pH, and significant lengths of time, ...incorporation of fluorophores, as seen in studies involving dopamine D2/D3 receptors (Allikalt et al., 2020...processes like mitochondrial dynamics. This process, involving mitochondrial homeostasis maintained through mechanisms...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression... of the most important applications for PA-FPs involve enhancing optical live imaging of proteins, organelles...and cells. Similar to FPs, PA-FPs allow for non-invasive labelling, but in contrast, PA-FPs offer a reduced...

  4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods...prime editing efficiency can vary widely — so its inventors kept optimizing it. Using a pooled CRISPRi screen...Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing...

  6. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...believe that science gets better when everyone gets involved. So join the conversation! Do you have a question...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...scientific progress made through years of public investment isn't lost. Universities and research institutions...benefit of everyone. We Want to Hear from You We invite researchers to share how these funding changes ...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...exciting way of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. ...Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....

  9. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... in learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and...

  10. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...Rights to all research results, modifications, and inventions created by the Recipient Right to file patent...patent applications on modifications or inventions Right to publish without editorial comment or review...

  12. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...

  13. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...information back. No team has all the answers – reinventing the wheel is inefficient. An effective team regularly...worry that it seems like "tattling" if others are involved. They may feel they are wasting their time. The...

  15. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...University. There are tons of ways you can get involved in science communication. In this second half ...science communication student group and get more involved with your local community. What do Vini and Amy...

  16. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...current postdocs will be academic principal investigators. She also pointed out promising new practices...Your PI”.   She focused her talk on the issues involved with the fact that the PhD and Postdoc advisors...

  17. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...plasmids to study the dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence...than discussing a new scientific discovery or invention, and sharing those ideas with others. This love...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are called...require a tracrRNA. sgRNA: “Single-guide RNAs” were invented by synthetic biologists. An sgRNA combines a crRNA...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... famous role as the “happy hormone,” it’s also involved in reinforcement learning, decision making, and...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...
Showing: 181 - 200 of 532 results