We narrowed to 810 results for: eng

...structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering, they developed...the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for... Nureki lab generated SpCas9-NG, a rationally engineered Cas9 with NG PAM compatibility, as the name implies...R.A. et al. PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368,290-296(2020...Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting ...Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant with PAM Flexibility History...

...proteins or TALE proteins, proteins had to be re-engineered for each gene, making wide-scale gene activation...gene activation: rather than requiring protein engineering for each loci, CRISPR/Cas systems only require...methods rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, ...Mediator (SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done... through creating a dCas9/VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These...activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research 41:...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https...

... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly limitations Embraced... assemblies that enable biosynthetic pathway engineering and optimization. However, current best practices... reduced these limitations through the use of engineered Type IIS restriction enzymes and careful choice...redesign was guided by both advances in the re-engineering of the original BsaI-HF® Type IIS restriction...In conjunction with BsaI-HFv2 (NEB #R3733), re-engineered to provide improved Golden Gate performance, ... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal Development...Development focusing on DNA cloning and assembly, New England Biolabs, Inc.         Eric Cantor is a molecular...

...recombinant protein purification and metabolic engineering. If instead you want to build genetic circuits...biology MoClo applications, but there are more! Engineering disease resistance through gene stacking, increasing...localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter for ...vectors, several delivery systems which can be engineered into the TUs, and even viral gene silencing vectors...kit has a focus on transformation and genome engineering. This is a specialized system with parts including...confined to that arena! The Mammalian Toolkit was engineered specifically for the types of expression and ...Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology...

...excitation wavelength (Salomonnson et al. 2012). They can also be excited efficiently at wavelengths between...development of cell sorters together with the engineering of fluorescently labelled antibodies made it ... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken... also important to finely tune the excitation wavelength as it has been shown that even a small incremental...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...

...Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic... SynBio. Chapter 3: Synthetic biology as the engineering of biology at the level of DNA using modular,...in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic production...lectures synthetic biology for the Department of Bioengineering. He graduated from Oxford University and then...supervision of Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one...undergraduate projects. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

...base editor when they realized that MPG could be engineered to act directly on unmodified G bases, resulting...Tong, Liu, et al., 2023). gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...., Qu, C., Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion...deaminase-free base editors for G-to-Y conversion by engineered glycosylase. National Science Review, 10(8), ... T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase. Nature Communications...

...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ...pEcCas/pEcgRNA system. This system works well for engineering single genome edits but the double strand breaks...interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system in two...compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine biosynthesis... systems to choose from. Ready for metabolic engineering with improved cargo dedicated to gene (cluster... optimizing strains with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute ... phenotypes and to design a new generation of engineered cells for green chemistry and biomedical applications...

...BioRender.com. How does it work? First, an engineered prime editing guide RNA (pegRNA) that both specifies... site and contains the desired edit (or edits) engages the prime editor protein. The prime editor consists...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...editing outcomes, including indels. Therefore, they engineered a dominant-negative mutant of the protein MLH1... RNA pseudoknots, calling this longer RNA an “engineered pegRNA” or epegRNA. Although the addition of ... which was made possible through their use of engineered virus-like particles. However, there’s still ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...

... protect samples during cryo-EM grid freezing Engineered and evolved optimizations for prime editing CHARM...https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved optimizations for prime editing By...applications! Delivering prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option...the donor sequence. The latest work evolves and engineers Bxb1 recombinase variants to optimize PASSIGE ..., P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like particles for transient delivery of...recombinases and prime editing. Nature Biomedical Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org... prion protein by AAV-mediated delivery of an engineered compact epigenetic editor. Science, 384(6703)...

...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...immediately applicable in DSB-induced genome engineering, it has biological importance for the repair ...CRISPR forum moderated by the Zhang lab CRISPR Challenges: Standardization and HDR CrispyCrunch Optimizing... (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. ...546; (2015). PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K...PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed. Enhancing..., et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR: a robust method for one-...

... problem when the excitation wavelength is shifted to longer wavelengths. Therefore, the use of bright...binds a synthetic, fluorescent analogue have been engineered (Thorn, 2017). These fluorescent analogues are...28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink ...above. The smallest tags are peptides that are engineered to bind specifically and with high affinity to...., 2017) may improve detection. Since longer wavelengths have better tissue penetration, (infra)red fluorescent...usually dim relative to fluorescent probes, recent engineering efforts have increased the brightness of these...fluorescent proteins, biosensors, and optogenetic tools engineered from phytochromes." Chemical reviews 117.9 (2017...

...theoretically, at least) fully and freely engage or disengage at will.  Informal education is education...typically ad hoc, for learners who can choose to engage or disengage with a very high level of independence, such...than average and are more likely to spend time engaging with science communication efforts.  Scientific...however, many others partner with formal learning to engage students at all levels.      Jargon is field-specific...space for people outside of the primary audience to engage if they so wish. (And let’s be honest: are you ...

...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from? ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...organism in the lab. Predicting lab-of-origin of engineered DNA This isn’t the only time researchers have...plasmid datasets) to predict the lab-of-origin of engineered DNA. Both of these groups have generated robust...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...Deep learning to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (2018): 3135....

...summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with...However, these approaches are often met with the challenges of regulatory bottlenecks and their scope is ... ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression of various...host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility...scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated the potential...By no means limited to the field of metabolic engineering, this is such a powerful and flexible technique... 1173-1183. (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with...

... versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam...editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the Cas9...PAM to recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for...recognizes NG rather than NGG. In addition to the engineered SpCas9, they also fused the SpCas9-NG with the... promoters and enhancers, or have attempted to engineer loops to create genomic interactions that wouldn...

....  The question for her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on...that they could be used by people with little bioengineering expertise? “This is the next step in high-throughput...Nature in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they...Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation..., unlike the mouse, we can’t rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” ...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD,...

... explore their Local Engagement Playbook for step-by-step guides on how to engage in your local community...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia...ranges from astronomy to bioethics to chemical engineering and everything in between. Plus, there are many...folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often there will be ...advocacy as a supplement to your career through engagement with your local or state governments. Most communities... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

...would work with our research team and software engineers to update our Lab Inventory Management System ...notice something: even though our scientists and engineers were attending the same meetings and hearing the...always knew from context what a “plate” was. Our engineers thought they knew.  As long as you have context...of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create a barcode...meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this guide was relatively easy...not have to communicate with a team of software engineers in your lab, you likely will need to write protocols... convey the same concept to Morgan, our senior engineer? The answer to these questions--which were real...

...Sainsbury Lab website or Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...overhangs to be efficiently assembled. Scientists can engineer unique enzyme recognition sites that flank their...cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11...Epub 2011 Apr 14. PubMed.   Golden Gate cloning. Engler C, Marillonnet S. Methods Mol Biol. 2014;1116:119...