Addgene

We narrowed to 207 results for: DAP

Showing: 181 - 200 of 207 results

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Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...
Plasmids 101: Biotinylation

Type

Blog Post

.... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

...mindedness, and the ability to accept feedback and adapt their mentoring styles to the mentee’s personality...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

...staining alternative. This method uses DNA stains like DAPI and Hoechst 33258 to stain all DNA. Some amount ...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

...closer look at three different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac...
Important Considerations When Using AAVs

Type

Blog Post

...Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...
Antibodies 101: Designing Your First Flow Panel

Type

Blog Post

...fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-...
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Type

Blog Post

...ICC) as a percentage of total cells in the field (DAPI). Using the ICC assay as a direct readout of tag...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

... a table listing some of critical differences (adapted from Zhou and Melton, 2008, [43]): Characteristic...
Antibody Validation for Flow Cytometry

Type

Blog Post

...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024. Once an antibody...
Optogenetics + CRISPR, Using Light to Control Genome Editing

Type

Blog Post

...CASANOVA system is versatile in that it can be adapted to regulate gene editing, turn on transcription...
Delivery Methods for Generating iPSCs

Type

Blog Post

...42. PubMed PMID: 21339765. Methods figures were adapted from this reference. 1. Malik, Nasir, and Mahendra...
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

Type

Blog Post

...functions more similarly to CRISPR/Cas9 systems adapted for eukaryotes in that Cas9-mediated cleavage induces...

Showing: 181 - 200 of 207 results

Results per page:

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Adeno-associated Viruses (AAVs) for Genome Editing

Plasmids 101: Biotinylation

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Treating Muscular Dystrophy with CRISPR Gene Editing

Antibodies 101: Multiplex Immunofluorescence

Technique: Probe Phage Genomes for Host Binding Proteins

Technologies Enabled by NanoLuc® Luciferase

Building Global Connections with the International Mentorship Program USA-EUROPE

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Plasmids 101: Using Transposons in the Lab

Important Considerations When Using AAVs

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Antibodies 101: Designing Your First Flow Panel

Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Antibody Validation for Flow Cytometry

Optogenetics + CRISPR, Using Light to Control Genome Editing

Delivery Methods for Generating iPSCs

CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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