...Triton X-100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1... of the 5 mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution...500 µL of 300 nM DAPI working solution for 10 min at RT in the dark. Remove the DAPI and dispose of it...Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100 Primary...solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section...

...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-... the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature...proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang...

...selection with carbenicillin; however it can be adapted for any antibiotic through the use of a selection...

...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...

...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...

...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection...

...protocol was established using 293T cells but can be adapted to alternative cell lines. Workflow Timeline Day...

...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last ...

...Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in a new window) Chomczynski P...