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  2. Typing CRISPR Systems

    Type
    Blog Post
    ...allowing for easy multiplexing. The double-stranded breaks caused by Cas12 create short 3’ overhangs, which...hypothesis, and even the time and money available. The breadth and diversity of CRISPR systems will no doubt keep...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Nihongaki, et al., 2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous...References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri ...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs...of cytosine to thymine without a double stranded break. However, the method was initially limited by off-target...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along ... simplifying experiments when additional cross-breeding steps are undesired or infeasible. Finally, an...
Showing: 181 - 200 of 373 results