Ligation Independent Cloning
Type
Protocol
... as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol...plasmid together through the transformation/replication process. LIC employs long overhangs to form a stable...product is then repaired during the replication cycle. Empty vectors for LIC typically employ type II restriction...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation...in the example shown here. The manufacturer of an LIC-specific vector will provide the homologous sequence...Protocol Step 1: Design Your Primers Primer design for LIC is often as simple as using the backbone manufacturer's... protocol . Search Addgene's collection of empty LIC cloning vectors...