...Viviana Gradinaru's lab at Caltech.... Viral Vector Packaging Service AAV Caltech Systemic Capsids Viral Vector...Vector Packaging Service: Caltech Systemic Capsids PHP.eB PHP.S PHP.V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22... from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in a new window) . As...decision tree was developed at the CLOVER Center at Caltech and can help guide your decision. Figure 1: Decision.... Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center (Link opens in a new window) and ...serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid ...

...qPCR Using SYBR Green Technology AAV Titration by qPCR Using SYBR Green Technology You may also like......particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. Protocols...genome-containing particles of an AAV prep using SYBR green technology. This protocol was validated using an internal...following protocol in your qPCR instrument using SYBR detection: 98 °C 3 min / 98 °C 15 sec / 58 °C 30 sec / read... A, Baiker A. Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...

...Eye protection and face shields, as needed Guidelines Your lab coat should be buttoned to protect your... personal protective equipment (PPE) for BSL-1 and BSL-2 labs. Protocols... Protocols Personal Protective Equipment...Equipment (PPE) for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Intro to...healthy humans. Many of the precautions are meant to protect individuals from potential accidents such as spills...Close-toed shoes and long pants should be worn to protect your legs and feet from potential spills. If wearing...shoes, cloth coverings or “booties” can be used to protect your feet. Pro-Tip If they are able, sometimes ...

...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...brainstorming new technological innovations. Let’s take a look at three historical techniques: southern blotting...classic techniques that allowed for many seminal scientific discoveries (5, 8). While newer techniques have...these, these techniques still have useful applications and have informed many current techniques. For example...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress... time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing classic...inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance. If a scientist...

...research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized...Luciferase-based tools are fantastic examples of technologies that can accelerate your research. The creation... Oplophorus luciferase by Promega’s Advanced Technologies group as well as evolution of the coelenterzine...RLuc reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR...I’ll cover two great applications of the NLuc technology. The first couples NLuc to fluorescent proteins...require external light sources. Unfortunately, detecting traditional luciferases’ dim light emissions requires...autofluorescence, and perturb biological phenomena. The technique of coupling FPs with Luciferases can overcome ...

...to two days.  Indirect or direct detection method Indirect detection is the most common way to run a western...choices to make when designing the experiment. What detection method? What membrane? What should you block with...for your experiment. But if you understand the technical aspects of a western blot, you'll be able to understand... so let's dive in!      Figure 1: The technical decisions to make when designing a western blot... western blot. It allows you to detect relatively low levels of antigen, while providing flexibility in... the more common chemiluminescent methods for detection (though the enzymes used for chemiluminescence...specific protein(s) of interest.  Determining linear detection range If you are quantifying your western blot...

...companies have real scientists answering their technical support questions (besides Addgene, I know both...emails and phones). We can’t be experts on every technique or experiment, but a picture of a gel or a sequencing... is answerable, particularly when it comes to technical questions. BUT, I do believe that scientists who... technical support...

...dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly accelerated...you think these technologies are going? What is the next hot molecular biology technology? Have you deposited...unique position to observe and quantify how new technologies are being disseminated through the scientific... plasmid sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander...

...alteration. Molecular cloning techniques are some of the most widely used techniques in the biological sciences...restriction cloning is one of the oldest of these techniques and is a multi-step process consisting of digestion...performed by many labs, a variety of other cloning techniques with higher efficiency and simpler procedures... still encounter several struggles with these techniques: unwanted mutations due to excessive PCR cycles...FastCloning is a simpler yet reliable cloning technique that was developed by Li, et al., in 2011. This... inserts. However, with any PCR-based cloning technique there is a chance of introducing an unwanted mutation...some tips for high efficiency cloning with this technique: Amplification efficiency is highest when using...

...get all the attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin... states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid,..., likely due to limitations of current means to detect RNA-protein interactions. To address this need,... Stanford created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin...but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID RaPID is similar...*s in the context of RaPID for their ability to detect a known RNA-protein interaction.  In as little ...a scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA...

...names for technical support roles. Examples are Technical Service Representative, Technical Support Specialist...these departments from technical support. Technical support careers in brief Technical support careers are...worked as a technical support specialist for 4 years. Read on for insights from my time in technical support...products successfully. In technical support you may have to answer technical enquiries such as: “Can I... areas of expertise in technical support positions. When applying for technical support positions, you...familiarize yourself with technical support. Even having personal experience with technical support through the... do well in a technical support role. What general characteristics do jobs in technical support have? ...

...ability to create a DNA detection platform called DETECTR. How does DETECTR work? DETECTR works similarly to...samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for simple...published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR has not been approved...nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements...nucleic acid detection however, either lack the sensitivity or the specificity to detect nucleic acids...CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous...atomolar levels, within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity...

... sectioning techniques due to a higher photon flux via the more efficient camera detector and lack of ...been a very slow imaging technique but recent advances in scanner technology and parallelization (7) have...sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the ...emerged as the dominant light microscopy contrast technique in modern biology (1). When performed correctly...block out of focus fluorescence from reaching a detector or restricting fluorescence to specific excitation...super-resolution (3). Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent...Microscopy was the first fluorescence microscopy technique to incorporate optical sectioning. Optical sectioning...

...toolkit based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a...continually impressed with the amazing plasmid technologies developed by our community of depositors. With...domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split" cas9...plasmids 62887 & 62888) Zetsche et al., Nat Biotechnol. 2015 Feb 2;33(2):139-42. doi: 10.1038/nbt.3149... system. The ease and flexibility of the LACE technology makes this system widely accessible for many ...imaging and inducible gene expression Brilliant technologies adopt basic biological concepts and employ them... the Weissman lab have teamed up and advanced technology in the fields of single molecule imaging and ...

...Addgene which allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These... Looking for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et...Jan 3;343(6166):84-7. Koike-Yusa et al., Nat Biotech. 2013. Dec 23.   Highly bright and extremely...new tool for peptide-peptide locking. The new technology is known as SpyLigase and is a protein domain...E690-7. For further reading about SpyLigase technology, read the Addgene interview with Mark Howarth...events occur in a physiological setting. Genetic techniques typically rely on overexpression or knock-down... and his team at the Institute of Science and Technology Austria, decided to take a novel approach to ...

...network with other technicians/company representatives. I got to talk with a technician from Neon Therapeutics...looking to try out a new technique or reagent! Racheal Komuhendo is a Lab Technician at Addgene who is interested...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ... we get many opportunities to learn from other biotech companies at workshops and can use what we learn...lab and office space shared by numerous emerging biotech companies. The space was modern with clean bold...heard about how LabCentral aims to enable emerging biotech companies, I was excited to go there because both... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...

...place to provide community protection. Toehold switches for pathogen detection In 2014, as postdoctoral...these combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic...also demonstrated our technology on active virus, as well as shown that we can detect the virus in a clinically...Harvard University), we developed two new enabling technologies for the field of synthetic biology. The first...for the rational design of RNA sensors that can detect virtually any RNA sequence (Green et al, 2014)....sensors to be used outside of the laboratory. The detection threshold for these RNA sequences (3 nM), however...blood, urine). Adapting toehold switches for Zika detection We and other members of the lab had been working...

...engineering through biotech. After graduating with a masters of science in Horticulutre Biotechnology, he did his...his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of...works as a lecturer in molecular genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe...sciences and agriculture 5:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10...Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's current...

... In Situ Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are...experience is focused on classical embryology techniques such as basic observation and dissections. Students...Students don’t usually perform more modern techniques used in genetics or molecular biology because the...through time or after genetic manipulation. The technique involves using synthetically made RNA (riboprobes...binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added to the tissue...labs to bypass these steps and focus on the WISH technique. The expression patterns of 11 different planarian...

...proteins to study. These are highly useful for the biotech industry, which is in the process of capitalizing...other modifications to their bases in order to protect their DNA from host cell nucleases (4). To further...event that genome sequencing can be done, this technique can also help pinpoint the genes within a sequenced...live cells The following is a description of a technique published by Simpson, Sacher, and Szymanski in...membranes well with PBST to remove unbound cells. To detect bound cells, simply lay each membrane on an agar...

... distributed plasmid technologies pSV-PGC1 - Ok, it's not actually a technology, but pSV-PGC1 was Addgene's...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10...genetically encoded calcium indicators were the hot technology of the year in our collection. CRISPR/Cas9 - ...Year of the CRISPR." The newest genome editing technology has been used and modified by so many labs and...

... complementary strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes its annealing...bacteriophage, which is not a common molecular biology technique. However, an advantage of this method is that ...

....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity...R​eporter un​LOCK​ing) technique Sensitivity: Single molecule per milliliter can be detected – in the zeptomolar...-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as low as 1.2 copies...visualization. In this technique, however, instead of using lateral flow strips to detect the cleavage of fluorescently...: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses FnCas9, a protein that...CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...

...approach you choose, it’s essential to consider the technical limitations of each approach and to follow best...

...do this.” Biotech startups are only successful if they are 100X better than current technology and get ...Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and Resume...developed during graduate school and turn it into a biotech company. Transitioning an idea from academia to...Sample6 is an enrichment-free bacterial pathogen detection system that uses synthetic biology to target and...concepts to engineer bacteriophages and use those to detect unwanted bacteria. How did graduate school help...a succesful entrepreneur? As a co-founder of a biotech startup, Mike explained, his career transitioned... the best product-market fit for this set of technologies, effectively communicating the advantages embodied...

...the limited technologies available. Over the past few years, however, remarkable technological advances ...revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists...mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics” was coined...fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting fusion gene... functional topography of the neural circuit architecture of the brain regions associated with motivated... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Deisseroth, Karl, et al. "Next-generation optical technologies for illuminating genetically targeted brain ...

...Branon et al. Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks ...often escape detection. The Alexander Green lab found a solution: a low-cost, cell-free detection assay that...isothermally, and detected through a toehold switch, an RNA sensor that can be used to detect the presence ...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be... have now applied the same directed evolution technique to the biotin ligase from which BioID was derived... and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, ...contributed by Izzy Mueller  Listen to the viral detection podcast segment As the primary cause for both...

... range of genome engineering techniques . Collectively, these techniques are referred to as CRISPR ( C...molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection platform... to SHERLOCKv2 to detect multiple targets. For example, Cas12 can be added to detect the presence of infectious...have an interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family...

...amplification through the indirect detection method. In the direct detection method, the signaling molecule.... Figure 3: A western blot detecting proteins using the indirect detection method, with a secondary conjugated...conjugated, it is often called the detection antibody. If using an indirect detection method, add a secondary antibody...microscopy techniques used, so be sure to read materials specific to your assay. Most IF techniques use a ...terms, summarize antibody production and storage techniques, and explain common applications. Science...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1...function is unknown Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving...

...Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably detect nucleic acids ...for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus detection using CRISPR-Cas13: Open-... ) for detection of SARS-CoV-2: A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV...Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR to detect...Reverse Transcriptases, etc. that may be useful for detection assays and more. Pooled Libraries : Pooled libraries... Blog: Finding nucleic acids with SHERLOCK and DETECTR . Feng Zhang's lab at the Broad Institute has utilized...detect coronavirus - A protocol for rapid detection of the 2019 novel coronavirus SARS-CoV-2 using CRISPR...