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Liu Lab MusCK Library A

Pooled Library

#174196

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK A contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

Pooled Library

#86538

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD Wuhan-Hu-1 SSM Library

Pooled Library

#1000000182

Depositor

Jesse Bloom

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

Pooled Library

#1000000095

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Eric Lander, David Sabatini

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Cancer and Apoptosis (h2), top 5 sgRNAs/gene

Pooled Library

#83972

Purpose

Human CRISPRi Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
Human Lentiviral sgRNA Library - Cell Cycle

Pooled Library

#51046

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
sgRNA pool for Streptococcus pneumoniae

Pooled Library

#170432

Purpose

CRISPR library targeting S. pneumoniae D39V

Depositor

Jan-Willem Veening

Article

Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

Expression

Bacterial

Use

CRISPR

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
pUC57kan-T7-mt-gRNA library

Pooled Library

#82480

Purpose

Pool of sgRNAs to remove mouse mitochondrial DNA reads in the sequencing of genomic libraries

Depositor

Wei Xie

Article

Wu et al Nature. 2016 Jun 30;534(7609):652-7.

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
Human Lentiviral sgRNA Library - Control

Pooled Library

#51048

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for control targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
scQers promoter control series (p027, p028, p029, p041, p042)

Pooled Library

#1000000239

Depositor

Jay Shendure

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
Human Transporter Knockout Library with UMIs

Pooled Library

#221409

Purpose

gRNA library to test the impact of transporters on biological processes

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only
p11-5’_10xN_PAM-site4 (RTW574)

Pooled Library

#160135

Purpose

Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
Human Genome-Wide Reduced Double-gRNA library

Pooled Library

#137999

Purpose

This gRNA library targets the human coding genome, aimed to reduce the scale of genome-wide perturbation screens up to fivefold compared to conventional methods, without sacrificing performance.

Depositor

Leopold Parts

Article

Peets et al bioRxiv 859652

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only
Pan-Druggable Cancer Library

Pooled Library

#182133

Purpose

All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only
Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set A

Pooled Library

#104090

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Stress and Proteostasis (h3), top 5 sgRNAs/gene

Pooled Library

#83973

Purpose

Human CRISPRi Pooled Library targeting stress and proteostasis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
mTF-CRISPRai

Pooled Library

#122131

Purpose

Expresses gRNAs for mouse transcription factor-CRISPR activation

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B

Pooled Library

#92386

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
lentiGuide-Puro-pKO-library

Pooled Library

#190744

Purpose

CRISPR/Cas9 paired-guide RNA library to delete proximal poly(A) sites in mouse melanoma.

Depositor

Robert Bradley

Article

Gabel et al Nat Commun. 2024 Feb 1;15(1):959. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only
Liu Lab MusCK Library B

Pooled Library

#174197

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK M_b contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - iPSC & HFF

Pooled Library

#86550

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B

Pooled Library

#92380

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only
BARBEKO sgRNA library

Pooled Library

#174163

Purpose

iBARed cytosine Base Editing-mediated gene KnockOut (BARBEKO) sgRNA library; This library leverages CRISPR cytosine base editors for genome-scale knockout screens.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
Human Lentiviral sgRNA Library - Ribosomal Proteins

Pooled Library

#51045

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Gene Expression (m5), top 5 sgRNAs/gene

Pooled Library

#83993

Purpose

Mouse CRISPRi Pooled Library targeting gene expression

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to HFF

Pooled Library

#86541

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
Human Lentiviral sgRNA Library - Unknown Function

Pooled Library

#51043

Purpose

Sub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function.

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
KSHV Tiling Library

Pooled Library

#180272

Purpose

sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

Depositor

Britt Glaunsinger

Article

Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

Expression

Mammalian

Species

Kaposi’s sarcoma-associated herpes virus

Use

Lentiviral

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD Omicron BA.1 SSM Library

Pooled Library

#1000000187

Depositor

Jesse Bloom

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD BA.2.86 SSM Library

Pooled Library

#1000000248

Depositor

Tyler Starr

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD XBB.1.5 SSM Library

Pooled Library

#1000000232

Depositor

Tyler Starr

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2_RBD_DMS_Library

Pooled Library

#1000000172

Depositor

Jesse Bloom

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only
Human Genome-wide Intron Tagging Library, Frame 1

Pooled Library

#216134

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
Human Genome-wide Intron Tagging Library, Frame 0

Pooled Library

#216133

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
hCRISPRi_dual_3_4

Pooled Library

#187247

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 5-8 in lentiCRISPRv2 backbone

Pooled Library

#75315

Purpose

Human sgRNA library in backbone lentiCRISPRv2 targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Cancer and Apoptosis (m2), top 5 sgRNAs/gene

Pooled Library

#83990

Purpose

Mouse CRISPRi Pooled Library targeting cancer and apoptosis genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to K562

Pooled Library

#86544

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
CHyMErA Large-Scale Exon Deletion hgRNA Library

Pooled Library

#209736

Purpose

CHyMErA Exon Deletion hgRNA Library allows identification of frame-preserving exons that affect cell fitness in human cells using the CHyMErA screening platform.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines

Pooled Library

#86539

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
Sctrans-PP

Pooled Library

#153101

Purpose

CRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiae

Depositor

Irina Borodina

Article

Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(

Expression

Yeast

Species

Saccharomyces cerevisiae

Use

CRISPR

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Mitochondria, Trafficking, and Motility (m4), top 5 sgRNAs/gene

Pooled Library

#83992

Purpose

Mouse CRISPRi Pooled Library targeting Mitochondria, Trafficking, and Motility genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
Human Whole Genome sgRNA iBAR Library

Pooled Library

#140633

Purpose

CRISPR pooled screen at high MOI by the embedded internal barcodes

Depositor

Wensheng Wei

Article

Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

Use

CRISPR and Lentiviral

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Liver CRISPR Knockout Library

Pooled Library

#192824

Purpose

CRISPR screening library containing ~72,000 sgRNAs

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
mCRISPRi-v2, Unassigned (m7), top 5 sgRNAs/gene

Pooled Library

#83995

Purpose

Mouse CRISPRi Pooled Library targeting unassigned genes

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
p11-3’_8xN_PAM-site2 (RTW555)

Pooled Library

#160133

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
Human CRISPR Poison Exon Knockout Library

Pooled Library

#138084

Purpose

The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

Depositor

Robert Bradley

Article

Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
Human Paralog Knockout Library (pgPEN)

Pooled Library

#171172

Purpose

CRISPR library targets human duplicate paralog gene pairs

Depositor

Alice Berger

Article

Parrish et al Cell Rep. 2021 Aug 31;36(9):109597. doi:

Expression

Mammalian

Species

Homo sapiens and Synthetic

Use

CRISPR and Lentiviral

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

Pooled Library

#138015

Purpose

This library can be used to investigate cardiac gene regulation. It targets transcriptional regulators that are expressed in mouse cardiomyocytes. A subset of GATA4 regulated genes are also included.

Depositor

William Pu

Article

VanDusen et al bioRxiv 2019, 808402v1; doi: https://doi

Species

Mus musculus

Use

AAV, CRISPR, and Cre/Lox

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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