Genome-scale CRISPR screening in a single mouse liver.
Keys HR, Knouse KA
Cell Genom. 2022 Dec 14;2(12):100217. doi: 10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
192824 | Mouse Liver CRISPR Knockout Library | CRISPR screening library containing ~72,000 sgRNAs |
192825 | pLentiCRISPRv2-Stuffer-HepmCherry | Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter |
192826 | pLentiCRISPRv2-Stuffer-HepmTurquoise2 | Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter |
192827 | pLentiCRISPRv2-sgAAVS1-HepmCherry | Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter |
192828 | pLentiCRISPRv2-sgAAVS1-HepmTurquoise2 | Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter |
192829 | pLentiCRISPRv2-sgMaob-HepmCherry | Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter |
192830 | pLentiCRISPRv2-sgLmnb2-HepmCherry | Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter |