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Genome-scale CRISPR screening in a single mouse liver.

Keys HR, Knouse KA
Cell Genom. 2022 Dec 14;2(12):100217. doi: 10.1016/j.xgen.2022.100217. Epub 2022 Nov 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
192824Mouse Liver CRISPR Knockout LibraryCRISPR screening library containing ~72,000 sgRNAs
192825pLentiCRISPRv2-Stuffer-HepmCherryContains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter
192826pLentiCRISPRv2-Stuffer-HepmTurquoise2Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter
192827pLentiCRISPRv2-sgAAVS1-HepmCherryExpresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter
192828pLentiCRISPRv2-sgAAVS1-HepmTurquoise2Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter
192829pLentiCRISPRv2-sgMaob-HepmCherryExpresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter
192830pLentiCRISPRv2-sgLmnb2-HepmCherryExpresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

Antibodies from Article