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pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

Plasmid

#175526

Purpose

Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag11 and Tomm20-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

Plasmid

#175525

Purpose

Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag10 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

Plasmid

#169338

Purpose

Expression of the LT-Fucci(CA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(CA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-3xrGBD

Plasmid

#176108

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LifeAct-HaloTag

Plasmid

#176105

Purpose

F-actin marker

Depositor

Dorus Gadella

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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tagSrc-HaloTag

Plasmid

#179141

Purpose

The tag is SsrA (AANDENY). Tag is inserted after N534 of Src.

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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WDR5-HaloTag

Plasmid

#200881

Purpose

Mammalian expression of human WDR5 with C-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

WDR5-HaloTag (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_DIO_R106W-HaloTag

Plasmid

#164063

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 R106W (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge