Live and Let Dye: Self-Labeling Protein Tags
Type
Blog Post
...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009).
HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...159
Trimpethoprim (TMP-tag)
Few
Easy
HaloTag
295
Chloralkane (CA)
Many
Easy
CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...protein of interest. For example, the Cohen lab used HaloTag for their “ticker tape” method for intracellular...tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular self-labeling...both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins...