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Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Codon Optimized Dysferlin
Plasmid#67878PurposeThe human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.DepositorInsertcodon optimized Dysferlin
UseUnspecifiedTagsNoExpressionMutationPromoterNo PromoterAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast
Plasmid#171951PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimizedPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + codon optimized Brn3a
Plasmid#86829Purposemammalian expression of the transcription factor Brn3aDepositorInsertBrn3a (POU4F1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast
Plasmid#171952PurposeAll-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-NLRP1 codon-optimized
Plasmid#166833PurposeMammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tagsDepositorInsertNLRP1 (NLRP1 Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mNeongreen-pGBD(PKN1 codon optimized)
Plasmid#129634PurposeVisualization of active RhoADepositorInsertRhoA binding doamin of PKN1(30 - 100) codon optimized
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ZmIAA29 (yeast codon optimized)
Plasmid#188441PurposeZea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimizationDepositorInsertAuxin Signaling Repressor IAA29 (yeast codon optimized)
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-codon optimized catalytically inactive PHGDH
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CycK-GFP (1-270AA) in Cilantro (Codon optimized)
Plasmid#169930PurposeOverexpression of 1-270AA CycK-GFP fusionDepositorInsertCCNK (CCNK Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ005 (AacC2c1 human codon optimized protein)
Plasmid#70171PurposeExpresses AacC2c1 human codon optimized proteinDepositorInsertAacC2c1 protein
UseTagsNLSExpressionMutationPromoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
Plasmid#184563PurposeCMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines. Codon-opti…PromoterCMVAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT
Plasmid#163991PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)
Plasmid#199567PurposeStable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform B (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagExpressionMutationcodon optimizedPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)
Plasmid#199570PurposeStable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform E (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagExpressionMutationcodon optimizedPromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform D (codon optimized)
Plasmid#199569PurposeStable and dox. inducible expression of Trim69 isoform D (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform D (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagExpressionMutationcodon optimizedPromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)
Plasmid#199568PurposeStable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transferDepositorInsertTrim69 isoform C (codon optimized) (TRIM69 Human)
UseRetroviral; Allows for puromycin selectionTagsFlagExpressionMutationcodon optimizedPromoterAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R
Plasmid#164002PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationE1461RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A
Plasmid#164001PurposeMammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imagingDepositorInsertNLRP1 (NLRP1 Human)
UseTagsmCherryExpressionMammalianMutationW1460APromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only