pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)
(Plasmid #199568)

• Purpose: Stable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transfer
• Depositing Labs: Andrea Cimarelli, Yuxin Song
• Publication: Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 10.1073/pnas.2211467119. Epub 2022 Oct 17.

Backbone

• Vector backbone: pRetroX-Tight-Pur
• Backbone manufacturer: Clontech
• Vector type: Retroviral ; Allows for Puromycin selection

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trim69 isoform C (codon optimized)
• Species: H. sapiens (human)
• Mutation: codon optimized
• Entrez Gene: TRIM69 (a.k.a. HSD-34, HSD34, RNF36, Trif)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry