Addgene

We narrowed to 617 results for: bifc

Showing: 1 - 20 of 617 results

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Use

Tags

BiFC C-terminal fragment

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Availability

Academic Institutions and Nonprofits only

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BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Use

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Use

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-bFOSDelataZipVC155

Plasmid

#22014

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosDelataZIPVC155

Use

Tags

HA

Expression

Mammalian

Mutation

deletion of residues 179-193 in bFos(118-211)

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-CC155

Plasmid

#22015

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

CC155

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-CrN173

Plasmid

#22016

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

CrN173

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VN

Plasmid

#110646

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

DDK and VN

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-4A-VN

Plasmid

#110647

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

DDK and VN

Expression

Mammalian

Mutation

C379A, C385A, C421A and C427A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VC

Plasmid

#110648

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

HA and VC

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-4A-VC

Plasmid

#110649

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Use

Tags

HA and VC

Expression

Mammalian

Mutation

C379A, C385A, C421A and C427A

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-NN

Plasmid

#105111

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Tags

nYFP/cYFP

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-NC

Plasmid

#105112

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Tags

cYFP-Myc and nYFP-HA

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CN

Plasmid

#105113

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Tags

cYFP-HA and nYFP-Myc

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CC

Plasmid

#105114

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Tags

cYFP-Myc/nYFP-HA

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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