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Purpose(Empty Backbone) ratiometric BiFC analysis in plants (transient transformation)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 105113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBiFCt-2in1-CN
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Vector typePlant Expression
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Selectable markerslacZ
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Tags
/ Fusion Proteins
- cYFP-HA (N terminal on backbone)
- nYFP-Myc (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- cYFP-HA (N terminal on backbone)
- nYFP-Myc (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBiFCt-2in1-CN was a gift from Christopher Grefen (Addgene plasmid # 105113 ; http://n2t.net/addgene:105113 ; RRID:Addgene_105113) -
For your References section:
A 2in1 cloning system enables ratiometric bimolecular fluorescence complementation (rBiFC). Grefen C, Blatt MR. Biotechniques. 2012 Oct 2;53(5):311-14. doi: 10.2144/000113941. 10.2144/000113941 PubMed 23066669