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pGGAselect

Plasmid

#195714

Purpose

pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSelect-2

Plasmid

#190242

Purpose

Encodes lacI-targeting gRNA for positive selection of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSelect-7

Plasmid

#190244

Purpose

Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSelect-9

Plasmid

#190245

Purpose

Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD5-frame_selector_0,1,2

Plasmid

#131152

Purpose

Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

CRISPaint frame selector sgRNAs 0,1,2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSELECT-HA-mFOXO1

Plasmid

#83308

Purpose

Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection marker

Depositor

Steven Abcouwer

Article

Kong et al Exp Eye Res. 2016 Aug 7;151:82-95. doi:

Insert

FoxO1 (Foxo1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

hEF1/HTLV prom

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_0

Plasmid

#127553

Purpose

Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 0

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_1

Plasmid

#127554

Purpose

Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_2

Plasmid

#127555

Purpose

Drosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 2

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (b')

Plasmid

#99837

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (b')

Use

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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env8 selectivity (d')

Plasmid

#99838

Purpose

mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

Insert

env8 selectivity (d')

Use

Tags

mNeonGreen

Expression

Bacterial

Mutation

A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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SELENOM CXXC

Plasmid

#86579

Purpose

Bacterial expression of SELENOM CXXC for protein production

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

SELENOM (SELENOM Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Sigal peptide was deleted; CXXC

Promoter

Tac

Available since

April 5, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pYAMTr2GCsgSc-SeLEU2

Plasmid

#224871

Purpose

Disruption of LEU2 genes in the Saccharomyces sense strict group species

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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SELENOM SXXS

Plasmid

#86580

Purpose

Bacterial expression of SELENOM SXXS for protein production

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

SELENOM (SELENOM Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Sigal peptide was deleted; SXXS

Promoter

Tac

Available since

April 5, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSELECT-HA-mFOXO1-D256

Plasmid

#83379

Purpose

expresses mouse FOXO1 with truncating mutation at AA 256 in mammalian cells

Depositor

Steven Abcouwer

Insert

Forkhead box O1 (Foxo1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

N256TAG (premature stop codon)

Promoter

EF-1a

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only
pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

Plasmid

#135814

Purpose

Fluorescent reporter for knock-in of TdTomato into the TH locus

Depositor

Lee Rubin, Tim Ahfeldt

Article

Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

Insert

TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)

Use

Tags

p2a-TdTomato

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SELE-Fc(DAPA)-AviTag-6xHis

Plasmid

#156908

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SELE (SELE Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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