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pCellFree_G03 PPARG

Plasmid

#67099

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PPARG (PPARG Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2

Plasmid

#78768

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPARG (Pparg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pMF-FU_TetO_GW_PPARG2

Plasmid

#193289

Purpose

Lentiviral construct that expresses a dox-inducible human PPARG2

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

PPARG2 (PPARG2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-PPARgamma

Plasmid

#16549

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR gamma (PPARG Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-terminal DNA-binding domain: aa 1-248

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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PGEX 4T-1-PPARgamma2

Plasmid

#78773

Purpose

To purify GST-tagged recombinant protein

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_PPARG_Hormone-recep

Plasmid

#109956

Purpose

Protein expression and purification of PPARG_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPARG_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCR2.1-ZFpparg

Plasmid

#44151

Purpose

This is intended for use in riboprobe synthesis, not for cloning.

Depositor

John Rawls

Article

Flynn EJ et al J Lipid Res. 2009 Aug;50(8):1641-52. doi

Insert

Peroxisome proliferator-activated receptor gamma (pparg Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
PCS4 3XFLAG-PPARgamma1

Plasmid

#78769

Purpose

To overexpress PPARgamma1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_1 (Pparg Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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PCS4 3XFLAG-PPARgamma2

Plasmid

#78770

Purpose

To overexpress PPARgamma2 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-PPARgamma2 2KR

Plasmid

#78772

Purpose

To overexpress PPARgamma2 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

K184A, K185A

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-PPARG

Plasmid

#192912

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

PPARG (PPARG Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe bleo human PPAR gamma 2

Plasmid

#11439

Depositor

Ronald Kahn

Insert

PPAR gamma 2 (PPARG Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Thierry Fournier, Severine Degrelle

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Use

Tags

secNluc

Expression

Mammalian

Mutation

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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PPRE-H2B-eGFP

Plasmid

#84393

Purpose

Fluorescent reporter for PPARgamma activity

Depositor

Thierry Fournier, Severine Degrelle

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE50

Plasmid

#90389

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

DR1

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE49

Plasmid

#90388

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

IRF3

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE39

Plasmid

#90379

Purpose

PPARg - PPARg homodimer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

RELB

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge