pPB-cT3G-cERP2-PPARG
(Plasmid
#192912)
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PurposeBarcoded piggybac transposon vector with Dox-inducible expression of PPARG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-CT3G-CERP2-MG
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Backbone manufacturerGeorge Church (Addgene plasmid # 175503)
- Backbone size w/o insert (bp) 6509
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPARG
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SpeciesH. sapiens (human)
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Insert Size (bp)1433
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Entrez GenePPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
- Promoter TET3G-miniCMV
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also expresses rTTA and puromycin acetyltransferase
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-cT3G-cERP2-PPARG was a gift from George Church (Addgene plasmid # 192912 ; http://n2t.net/addgene:192912 ; RRID:Addgene_192912) -
For your References section:
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression. Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM. Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291. 10.7554/eLife.83291 PubMed 36803359