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PurposeFluorescent reporter for PPARgamma activity
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 84393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneH2B-GFP
- Backbone size w/o insert (bp) 5100
- Total vector size (bp) 6000
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Modifications to backboneCMV promotor was removed and replaced by PPRE sequence
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPARG response element
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Alt namePPRE
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Alt nameApoCIII promotor
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Entrez GenePPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
- Promoter PPRE
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Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AseI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer PPRE-AseI
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGeoff Wahl (Addgene plasmid # 11680)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PPRE-H2B-eGFP was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 84393 ; http://n2t.net/addgene:84393 ; RRID:Addgene_84393) -
For your References section:
New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. Degrelle SA, Shoaito H, Fournier T. PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. 10.1155/2017/6139107 PubMed 29225614