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pCFJ151 - ttTi5605_MCS
Plasmid#19330DepositorArticleInsertttTi5605 targeting region
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJA42
Plasmid#59930PurposegRNA for cleavage at rol-6(su1006) locus in C elegansDepositorInsertrol-6(su1006) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD317 (Pmyo-2::GFP + SEC)
Plasmid#73344PurposeSEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsDepositorInsertPmyo-2::GFP + SEC
UseCRISPR and Cre/LoxTagsGFPExpressionWormMutationPromotermyo-2Available sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#205
Plasmid#110881PurposeExpresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationchanged Asp 448 to AsnPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD268
Plasmid#132523PurposemNG^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mNGExpressionWormMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150 - pDESTttTi5605[R4-R3]
Plasmid#19329DepositorArticleInsertpDESTttTi5605[R4-R3]
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDD288
Plasmid#137017PurposemNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mNGExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
UseTagsExpressionWormMutationPromoterpie-1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-GFP(dpiRNA)::CDK-1
Plasmid#107938Purposecdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertGFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
UseTagsExpressionWormMutationPromotercdk-1Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM3774
Plasmid#154117PurposetetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1DepositorInserttetO 7X::∆mec-7p::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ782 - HygroR
Plasmid#190933PurposeHygromycin resistance co-injection markerDepositorInsertHygromycin resistance gene
UseTagsExpressionWormMutationPromoterrps-0Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ909
Plasmid#44480DepositorInsertMinimal Mos1, unc-119, MCS
UseMultiple cloning site vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only
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