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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34834 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
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Modifications to backbonepBluescript was converted into pAD12, with the introduction of T7 promoters at both sides of MCS.
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Vector typeBacterial Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedaf-2
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SpeciesC. elegans (nematode)
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Insert Size (bp)2000
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Entrez Genedaf-2 (a.k.a. CELE_Y55D5A.5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer M13F(-20)
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAndrew Dillin and Cynthia Kenyon, UCSF.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD48-daf-2 RNAi was a gift from Cynthia Kenyon (Addgene plasmid # 34834 ; http://n2t.net/addgene:34834 ; RRID:Addgene_34834) -
For your References section:
Timing requirements for insulin/IGF-1 signaling in C. elegans. Dillin A, Crawford DK, Kenyon C. Science. 2002 Oct 25;298(5594):830-4. 10.1126/science.1074240 PubMed 12399591