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Plasmid#19487DepositorInsertHSPA8 (HSPA8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMT-HuC:Dendra2
Plasmid#80904PurposeInjection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2DepositorInsertDendra2 (elavl3 Synthetic)
UseZebrafish expressionTagsExpressionMutationPromoterHuCAvailable sinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT
Plasmid#60814Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFP-NLS (SV40)ExpressionMammalianMutationPromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE2-TetO-hSOX2
Plasmid#206388PurposeExpresses human SOX2 in mammalian cells, for lentivirus generation.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)
Plasmid#164184PurposeFreeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Zeo_cOVA_T2A_mStrawberry
Plasmid#161736PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-mCherry
Plasmid#159742PurposeExpresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-Fbx15-cont2
Plasmid#27081DepositorInsertFBXO15 (FBXO15 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6 SgRNA RBM20
Plasmid#162735PurposeMammalian expressionDepositorInsertSpacer sequence for RBM20
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6*)-mCherry
Plasmid#168048PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101* and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJA1
Plasmid#19491DepositorInsertDNAJA1 (DNAJA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_alphaTubulin_C1
Plasmid#85047PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HtrA2-EGFP
Plasmid#14121DepositorInsertHtrA2 (HTRA2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-S
Plasmid#172426PurposeExpression of full-length, human GIRK2 with C-terminal StrepTag IIDepositorInsertGIRK2 (KCNJ6 Human)
UseTagsStreptag IIExpressionInsectMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_VSVgSS-diCR2
Plasmid#207324PurposeExpression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infectionDepositorInsertdiCR2 (LDLR Human)
UseTagsExpressionMammalianMutationfragment of LDL-R dimerizedPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEFL EGFP-TEADi
Plasmid#140144PurposeTEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.DepositorInsertEGFP TEADi
UseTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only