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PurposeIn vivo visualization of microtubules (can be used for colocalization studies)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmScarlet-i_C1
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Backbone manufacturerGadella Lab
- Backbone size w/o insert (bp) 4674
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAlpha-Tubulin
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Alt nameTUBA1B
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SpeciesH. sapiens (human)
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Insert Size (bp)1350
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Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet-i (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer AGGTCTATATAAGCAGAGC
- 3′ sequencing primer TCTACAAATGTGGTATGGC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmScarlet-i_alphaTubulin_C1 was a gift from Dorus Gadella (Addgene plasmid # 85047 ; http://n2t.net/addgene:85047 ; RRID:Addgene_85047) -
For your References section:
mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. 10.1038/nmeth.4074 PubMed 27869816