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Plasmid#121687PurposepMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1L2
Plasmid#113735PurposeGateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CAG.Pro
Plasmid#79490PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoterDepositorInsertCAG Promoter
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCAG PromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
rtTA and TagBFP cassettes
Plasmid#62452PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::PuroR-bGHpA
Plasmid#62439PurposeMXS_chaining vector with CMV::PuroR-bGHpADepositorInsertresistance cassette against Puromycin with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290
Plasmid#129670PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationAacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-mCherry
Plasmid#79509PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDSDepositorInsertmCherry RFP
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP2-1st&2nd WW mutant
Plasmid#19048DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 changed to Alanine,and Proline 202…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3E-ML1N*2
Plasmid#67678PurposeMultisite gateway entry clone for adding C-terminal fusions of ML1N*2DepositorInsertML1N*2
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TCF7
Plasmid#79498PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDSDepositorInsertTCF7 (TCF7 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-SOS
Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4A02
Plasmid#71021PurposeGateway entry cloneDepositorInsertUbe3a (Ube3a Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292
Plasmid#129672PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationAaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(V266E)
Plasmid#90089PurposeExpresses caspase 3 V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationV266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf9b
Plasmid#158378PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf9b
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-P2A-TurboID-NS
Plasmid#166568PurposeMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYNDepositorInsertmKate2-P2A-TurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2 Chfr (1014)
Plasmid#61853Purposemammalian expresion of Chfr fused to EGFPDepositorInsertChfr (CHFR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, K80DPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only