pET21b-Caspase-3(V266E)
(Plasmid
#90089)
-
PurposeExpresses caspase 3 V266E mutant in bacterial cells
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 90089 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET21b
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCaspase 3
-
SpeciesH. sapiens (human)
-
MutationV266E
-
Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
-
Tag
/ Fusion Protein
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET21b-Caspase-3(V266E) was a gift from Clay Clark (Addgene plasmid # 90089 ; http://n2t.net/addgene:90089 ; RRID:Addgene_90089) -
For your References section:
Mutations in the procaspase-3 dimer interface affect the activity of the zymogen. Pop C, Feeney B, Tripathy A, Clark AC. Biochemistry. 2003 Oct 28;42(42):12311-20. 10.1021/bi034999p PubMed 14567692