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Plasmid#165599PurposeGoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmidInsertTaU6 promoter::LacZ::sgRNA scaffold
UseCRISPR and Synthetic Biology; Sgrna acceptor with…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK087
Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM117
Plasmid#127212PurposeBeYDV replicon with constitutive Luc expression, sgRNA targeting PDSDepositorInsertLuc, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCM935
Plasmid#58202PurposeExpresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.DepositorInsertunc-22 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM808
Plasmid#134181PurposeLentiviral sgRNA plasmid with blasticidin resistanceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-GFP-SFPQ
Plasmid#97084PurposeEncodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.DepositorInsertsgRNA GFP-SFPQ
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH113
Plasmid#128210Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23117Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH49
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH51
Plasmid#128213Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK093
Plasmid#171365PurposeVector part (LVL1) from the Fungal Modular Cloning ToolKit.DepositorInsertsgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9-J23109
Plasmid#113152PurposePlasmid constitutively expressing eSpCas9 proteinDepositorInserteSpCas9
UseTagsExpressionBacterialMutationK810A & K1003A & R1060APromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.sfGFP-RepEntry.iPAC
Plasmid#100893PurposeLentiviral CRISPR-Cas9 ReporterDepositorInsertssfGFP
PAC
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
BC1364-mouse U6-sgH2B
Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorInsertH2B targeting sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
peSpdCas9-J23109
Plasmid#113153PurposePlasmid constitutively expressing eSpdCas9 proteinDepositorInserteSpdCas9
UseTagsExpressionBacterialMutationD10A & K810A & H840A & K1003A & R…PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only