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Plasmid#155243Purposefor mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19DepositorInsertUSP19 (delta1-1631) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER deltaN2
Plasmid#155242Purposefor mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19DepositorInsertUSP19 (delta1-1088) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER deltaN1
Plasmid#155241Purposefor mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19DepositorInsertUSP19 (delta1-536) (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CYT
Plasmid#155238Purposefor mammalian overexpression of the cytoplasmic isoform of rat USP19DepositorInsertUSP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIS-rPALcc
Plasmid#145804Purposeexpresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
UseTagsExpressionMammalianMutationchanged serine 767 to alanine to prevent glycosyl…PromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr2
Plasmid#137798PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr1
Plasmid#137795PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin 1 (Arrb1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR DRY->AAY-p2A-mCherry
Plasmid#137784PurposeVisualization of the At1aR DRY mutantDepositorInsertRat At1a receptor; DRY mutant (Agtr1a Rat)
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR DRY->AAY-mCherry
Plasmid#137783PurposeVisualization of the At1aR DRY mutantDepositorInsertRat At1a receptor; DRY mutant (Agtr1a Rat)
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_015
Plasmid#123796PurposeEncodes rat Connexin 26 as a Type 3b part to be used in the MTK systemDepositorInsertCx 26 (rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_017
Plasmid#123798PurposeEncodes rat Connexin 43 as a Type 3b part to be used in the MTK systemDepositorInsertCx43 (rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-P22S-EGFP
Plasmid#132594PurposeExpresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutationDepositorInsertNEFL (Nefl Rat)
UseTagsEGFPExpressionMammalianMutation64C>A, g5C>G, Pro22SerPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorInsertshNts(2) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorInsertshNts(1) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(2) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ rat MYO1D
Plasmid#118419PurposeFor mammalian expression of rat myosin 1DDepositorInsertrat myosin 1D (Myo1d Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>Fab-srt-his
Plasmid#124810PurposeHDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.DepositorInsertrat IgG2a Hinge-G4S-Sortag-Histag
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterialMutationPromoterpUCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73AGW (F2)
Plasmid#74039Purposelentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationS73APromoterH1Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only