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pCW-Cas9-2A-EGFP
Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotertTRE, hPGKAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
UseTagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-eGFP-CCR5TC
Plasmid#46296DepositorInsertGPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48R
Plasmid#17604PurposeMammalian expression of HA tagged ubiquitin K48RDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK48R (expected to disrupt G76-K48 ubiquitin chain…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
AP2u2-mCherry
Plasmid#27672DepositorInsertAp2m1 (Ap2m1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2/LTR7-GFP
Plasmid#62541PurposeLTR7-driving reporterDepositorInsertLTR7-GFP
UseSleeping beauty transposonTagsExpressionMammalianMutationPromoterLTR7Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIN-PAmCherry-KFERQ-NE
Plasmid#102365PurposeLentiviral overexpression of PA-mCherry-KFERQ fusionDepositorInsertsPA-mCherry
KFERQ peptide
UseLentiviralTagsNEExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24
Plasmid#28138DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-C1
Plasmid#54546PurposeLocalization: C1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA_Cloning Vector
Plasmid#41824PurposeAn empty gRNA expression vector, used to create a gRNA to a specific sequence.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsAflII site for Gibson AssemblyExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6gRNA1-U6gRNA2-TnT-Cre
Plasmid#87682PurposeAAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.DepositorInsertsgRNA1
gRNA2
Cre
UseAAVTagsExpressionMutationPromoterU6 and cTnTAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-N1
Plasmid#54843PurposeLocalization: N1 Cloning Vector, Excitation: 434, Emission: 474DepositorTypeEmpty backboneUseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG_smFP V5
Plasmid#59758Purposeencodes multiple copies of an epitope tag V5DepositorInsertsmFP _V5
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
MLRV
Plasmid#118069PurposeMulti-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reportersDepositorInsertsTB:5xNF-κβ:RedF:bGHpA
TB:4xTGF-β:FLuc:bGHpA
TB:5xE-box:Renilla:bGHpA
TB:2xp53:NLuc:bGHpA
TB:6xAP-1:GrRenilla:bGHpA
CMV:ELuc:bGHpA
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoter2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only
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