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pUAS-ERK-YFP

Plasmid

#42076

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila ERK ortholog (rl Fly)

Use

Tags

YFP

Expression

Insect

Mutation

Promoter

Hsp70

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pFB2B

Plasmid

#195284

Purpose

Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Parkin-IRES-zeo

Plasmid

#61727

Purpose

Expression of human Parkin fused to mCherry

Depositor

Stephen Tait, Douglas Green

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFB2C

Plasmid

#195285

Purpose

Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A Hygro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Article

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Entry vector

Tags

Expression

Mutation

silent mutation that renders it resistant to siRN…

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin 1

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

Plasmid

#193719

Purpose

Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)

Use

Tags

2xHA-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

Plasmid

#193721

Purpose

Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3G919C

Plasmid

#120255

Purpose

Expression of ATP3-7 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G919 changed to C; Alanine 307 changed to Proline

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-PIAL2M-sim1

Plasmid

#67908

Purpose

Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

Depositor

Andreas Bachmair

Article

Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

Insert

PIAL2 (AT5G41580 Mustard Weed)

Use

Tags

MBP

Expression

Bacterial

Mutation

E281-A496; VEDL 425-428 AAA

Promoter

P-lac

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-Rab5A

Plasmid

#28043

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab5A (RAB5A Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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mito-V5-APEX2

Plasmid

#72480

Purpose

Expresses APEX2 in mitochondria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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Golgi-mEGFP

Plasmid

#182877

Purpose

Localization: Gal-T - Golgi Complex

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

Golgi-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAN-PA4-RFP

Plasmid

#62250

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pA4

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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