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Plasmid#220082PurposePA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear exportDepositorInsertSpo20 (SPO20 Budding Yeast)
UseTagsX. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…ExpressionMammalianMutationamino acids 51-91, tandem dimerPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
Src(WT)-SsrA(Y7I)-mCer3
Plasmid#220998PurposeThis is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminalDepositorInsertSrc, SsrA, mCerulean3 (Src Mouse)
UseTagsmCerulean3ExpressionMammalianMutationY7I in SsrAPromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
NET5s pEGFP-N2 (1154)
Plasmid#62034Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET5s (TMEM201 Human)
UseTagsEGFPExpressionMammalianMutationshort formPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-DsRed-cNLS
Plasmid#138533PurposeLentiviral inducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-mCherry-cNLS
Plasmid#138531PurposeLentiviral inducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)
Plasmid#192442PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.DepositorInsertmCherry-TSC2(S939A,T1439A) (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-NL1
Plasmid#173007PurposeFor light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.DepositorInsertDHFR-GFP-NL1
UseTagsDHFR and EGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-XS-VWF
Plasmid#64847PurposeExpresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminusDepositorInsertVenus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Synthetic, Human)
UseTags7X His, Cerulean, and VenusExpressionBacterialMutationPromoterT7Available sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
WFS1 pEGFP-N2 (1472)
Plasmid#62051Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertWFS1 (WFS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
P9_TEVs_cLuc
Plasmid#119303PurposeExpresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logicDepositorInsertsplit cLuc connected to P9 coil via TEVs cleavable linker
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tmem38A pEGFP-N2 (1401)
Plasmid#62050Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem38A (TMEM38A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only