-
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-jGCaMP7f-WPRE
Plasmid#203848PurposeExpression of GCaMP7f in CaMKII expressing cellsDepositorInsertjGCaMP7f
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti EF1a-FLAG-dCas9-VPR
Plasmid#114195PurposeLentivirus compatible dCas9-VPR construct driven by the EF1a promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-GtACR1_C102A-EYFP
Plasmid#119196PurposeStep-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open stateDepositorInsertAnion channelrhodopsin GtACR1 step-function mutant C102A
UseLentiviralTagsEYFPExpressionMutationchanged cysteine 102 to alaninePromoterUbiquitinAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_GPHN.FingR-mKate2-IL2RGTC
Plasmid#46297DepositorInsertGPHN.FingR-mKate2-IL2RGTC (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGW_PSD95.FingR-YFP
Plasmid#46299DepositorInsertPSD95.FingR (DLG4 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta WT GFP
Plasmid#128820PurposeTo visualize Doc2beta WT in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-Flex-TC66T
Plasmid#48331PurposeCre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVADepositorInsertTVA receptor mCherry fusion with the 66T mutation
UseAAV and Cre/LoxTagsmCherry fusion to TVA receptorExpressionMammalianMutation66TPromoterCAGAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY26
Plasmid#191031Purposecho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.3-2.6k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY39
Plasmid#191043Purposeacr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(2.1k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGW_GPHN.FingR-YFP
Plasmid#46298DepositorInsertGPHN.FingR (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
Plasmid#83491Purposecre-dependent DN-AMPK under EF1a promoterDepositorInsertDN-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationK45RPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEY43
Plasmid#191047Purposeeat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInserteat-4(prom6-1) (eat-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB
Plasmid#137001PurposemEGFP-CREB donor for G-CREBDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
NgΔIQ FUNgDIQGW
Plasmid#92232PurposeLentiviral expression of human Ng with deletion mutationDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdelta IQPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCyRFP2-CREB
Plasmid#137003PurposemCyRFP2-CREB donor for R-CREBDepositorInsertCREB1 (Creb1 Rat)
UseTagsmCyRFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha WT
Plasmid#128812PurposeTo make virus expressing Doc2alpha WTDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta WT
Plasmid#128814PurposeTo make virus expressing Doc2beta WTDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha WT GFP
Plasmid#128819PurposeTo visualize Doc2alpha WT in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-0.27kb-EGFP
Plasmid#153185PurposeTruncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
NgOE FUNgGW
Plasmid#92231PurposeLentiviral expression of human NgDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-KIX-mCherry
Plasmid#137004PurposemCherry-KIX acceptor for G-CREBDepositorInsertKIX domain (Crebbp Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mMaroon-KIX-mMaroon
Plasmid#137005PurposemMaroon-KIX acceptor for R-CREBDepositorInsertKIX domain (Crebbp Rat)
UseTagsmMaroonExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-nArgBP2ΔNSE
Plasmid#74515PurposeExpress GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.DepositorInsertnArgBP2delNSE (Sorbs2 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Cav1 in situ probe
Plasmid#45624DepositorInsertCav1 in situ probe (Cav1 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1472-2124 of AB029929.1PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEY61
Plasmid#191058Purposeacr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(3.7k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-0.66kb-EGFP
Plasmid#153165PurposeTruncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Ngn1
Plasmid#118589PurposeExpresses Ngn1 from the TRE3G promoterDepositorInsertNgn1 (Neurog1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-1.03kb-EGFP
Plasmid#153164PurposeTruncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCit-Snap25b D179K A199R
Plasmid#53259PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationD179K and A199RPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Btg1
Plasmid#45865DepositorInsertBtg1 in situ probe (Btg1 Mouse)
UseIn situTagsExpressionMutationcontains bp# 1004-1638 of BC006834.1PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Chn2 in situ probe
Plasmid#45625DepositorInsertChn2 in situ probe (Chn2 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 2212-2619 of BC051139.1PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Inhba
Plasmid#45868DepositorInsertInhba in situ probe (Inhba Mouse)
UseIn situTagsExpressionMutationnt 428-933 from Inhba mRNA (NM_008380.1)PromoterAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Human USP7_H456A-3X FLAG
Plasmid#225335PurposeLentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationH456APromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT-C34-L
Plasmid#223967PurposeAnti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.DepositorInsertVariable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO-mCherry_Dicer gRNA
Plasmid#215279PurposeExpresses Dicer gRNA in a Cre dependent mcherry vectorDepositorInsertDicer control gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO-mCherry_DicerTTT gRNA
Plasmid#215280PurposeExpresses Dicer Control gRNA in a Cre dependent mcherry vectorDepositorInsertDicer control gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA
Plasmid#215282PurposeExpresses Grin1 Control gRNA in a Cre dependent GCaMP8f vectorDepositorInsertGrin1 control gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
Plasmid#217514PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217515PurposeEncodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217513PurposeEncodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Plekha5E10 WT
Plasmid#209576Purposeminigene construct for Plekha5 E10 alternative splicingDepositorInsertPlekha5 (Plekha5 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-3xFLAG-bII spectrin (repeats 13-15)
Plasmid#213729PurposeMammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.DepositorInsertbII spectrin (repeats 13-15) (Spnb3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only