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Plasmid#178728PurposeAAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)DepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-iC++-EYFP
Plasmid#137157PurposeIntersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-iC++-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-NpHR3.3-EYFP
Plasmid#137152PurposeIntersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-NpHR3.3-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-sRGECO
Plasmid#137126PurposeIntersectional viral expression of sRGECO in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE217DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet
Plasmid#137161PurposeIntersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-ChRmine-p2a-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationoScarlet E95DPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP
Plasmid#137131PurposeIntersectional viral expression of BFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV H2B iRFP
Plasmid#206253PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertH2B iRFP713
UseRecombinant baculovirus production, multimate/gat…TagsiRFP713ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Coff/FonEYFP
Plasmid#55652PurposeCre-off/Flp-on EYFP under the Synapsin promoterDepositorInserteYFP with introns
UseAAV; Cre off/flp on eyfpTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V2
Plasmid#127848Purposenon-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL
Plasmid#111397PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mCherryExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B4
Plasmid#127849Purposenon-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B4 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-oScarlet
Plasmid#137136PurposeIntersectional viral expression of oScarlet in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationE95DPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA
Plasmid#178826PurposeCre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoterDepositorInsertSomQuasAr6a_EGFP-P2A-somCheRiff_HA
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP; HAExpressionMammalianMutationPromoterhSynAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS4
Plasmid#170855PurposeAAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Foff EYFP
Plasmid#55651PurposeCre-on/Flp-off EYFP under the Synapsin promoterDepositorInserteYFP with introns
UseAAV; Cre on/flp off eyfpTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-BFP
Plasmid#137129PurposeIntersectional viral expression of BFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLPo-Myc
Plasmid#124641PurposeAAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoterDepositorInsertFLPo
UseAAV and Cre/LoxTagsmyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6F
Plasmid#137124PurposeIntersectional viral expression of GCaMP6F in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2-mCherry
Plasmid#137144PurposeIntersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-ChR2-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationH134RPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL
Plasmid#111394PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only