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Plasmid#158184PurposeGateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK4 (DYRK4 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CAMK1G_IDG-K
Plasmid#158168PurposeGateway destination clone of CAMK1G (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMK1G (CAMK1G Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140168PurposeExpresses a regulatable secretory cargo for Tetrahymena thermophilaDepositorInsertssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY921
Plasmid#135092PurposeTranscriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
UseTagsGFP and Synthetic IntronExpressionWormMutationPromoterPacr-2Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY847
Plasmid#135093PurposeTranscriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegansDepositorInsertPacr-2
UseTagsmcherry and unc-10 3'UTRExpressionWormMutationPromoterPacr-2Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CCG 316
Plasmid#131166PurposeFor ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
kank1a_pJet1.2
Plasmid#121978Purposefor generating kank1a riboprobes for WISH in zebrafishDepositorInsertkank1a (si:dkey-146l2.1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-WT
Plasmid#114065Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsGSTExpressionInsectMutationnonePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SsoPCNA3
Plasmid#107069PurposeExpresses S. solfataricus PCNA3 fused to MBP in E. coliDepositorInsertS. solfataricus PCNA3
UseTags6xHis-thrombin cleavage site and MBPExpressionBacterialMutationPromotertac promoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
P415 δCLD+Ret2 2α
Plasmid#73878PurposeSwapped helicesDepositorInsertδCLD+Ret2 2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD+δC2α
Plasmid#73877PurposeSwapped helicesDepositorInsertRet2LD+δC2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG
Plasmid#91945PurposeBacterial expression of maize SAE1 and truncated SAE2DepositorInsertsHA-ZmSAE1
ZmSAE2-FLAG
UseTagsFLAG and HAExpressionBacterialMutationTruncation of ZmSAE2 to mimic a splice variantPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTX203
Plasmid#89267PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02DepositorInsertOsDEP1-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterMaize Ubiquitin1 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only