YIplac204-pOst1-ESCargo(Orange)
(Plasmid
#140157)
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PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYIplac204
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
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SpeciesSynthetic
- Promoter TPI1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YIplac204-pOst1-ESCargo(Orange) was a gift from Benjamin Glick (Addgene plasmid # 140157 ; http://n2t.net/addgene:140157 ; RRID:Addgene_140157) -
For your References section:
ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Casler JC, Zajac AL, Valbuena FM, Sparvoli D, Jeyifous O, Turkewitz AP, Horne-Badovinac S, Green WN, Glick BS. Mol Biol Cell. 2020 Oct 28:mbcE20090591. doi: 10.1091/mbc.E20-09-0591. 10.1091/mbc.E20-09-0591 PubMed 33112725