Skip to main content
Addgene
Showing: 1861 - 1880 of 1967 results

These results may also be relevant.

  1. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48348-plasmid-map-sequence-id-70559

    Enlarge

  4. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #165492
    Purpose
    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    darkVenus
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165492-plasmid-map-sequence-id-330311

    Enlarge

  7. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid
    #75406
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75406-plasmid-map-sequence-id-146004

    Enlarge

  9. pcDNA3 RL3m6L2

    Plasmid
    #109366
    Purpose
    Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag
    Depositor
    Robert Lucas
    Article
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Insert
    Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
    Use
    Tags
    1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109366-plasmid-map-sequence-id-210142

    Enlarge

  16. pLKO.1-shGBP1.2.mKO2

    Plasmid
    #85210
    Purpose
    TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85210-plasmid-map-sequence-id-169782

    Enlarge

  18. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

    Enlarge

Showing: 1861 - 1880 of 1967 results