Showing: 1841 - 1860 of 13889 results
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Plasmid#128763PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #2 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3
Plasmid#128764PurposeExpresses DIS3L2 gRNA for CRISPRiDepositorInsertDIS3L2 sgRNA #3 (DIS3L2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin
Plasmid#134864PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertemerin (EMD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDuBir-Lbu-dCas13a-avitag
Plasmid#100817PurposeDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13aDepositorInsertLbu_Cas13a_R472A_H477A_R1048A_H1053A
UseTagsAviTag and His6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-tagRFP-FLAG-cGAS
Plasmid#86676PurposeExpresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInserttagRFP-FLAG-cGAS
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-mCherry-cNLS
Plasmid#138531PurposeLentiviral inducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-tdTomato-cNLS
Plasmid#138532PurposeLentiviral inducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-DsRed-cNLS
Plasmid#138533PurposeLentiviral inducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseLentiviralTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE-CMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectMutationPromoterFused Actin-HSP70AvailabilityAcademic Institutions and Nonprofits only -
CKAP2 pHAT
Plasmid#175946PurposeBacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorInsertCKAP2 (Ckap2 Mouse)
UseTags6-His and StrepTagIIExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
CKAP2-mNeonGreen pHAT
Plasmid#175947PurposeBacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorInsertCKAP2 (Ckap2 Mouse)
UseTags6His, StrepTagII, and mNeonGreenExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)
Plasmid#172326PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1-alphaAvailabilityAcademic Institutions and Nonprofits only
Showing: 1841 - 1860 of 13889 results