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Plasmid#171464PurposeExpresses mChF-Cav1s, a FiCE of iCMM, in mammalian cellsDepositorInsertmCherry-FKBP12-Caveolin1(61-178 aa)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RABIF-FLAG
Plasmid#135558PurposeExpress human RABIF in mammalian cells, FLAG at N terminalDepositorInsertRABIF (RABIF Human)
UseTagsFlagExpressionInsectMutationPromoterT7 promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q769gp120-8his
Plasmid#100929PurposeMammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strainDepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306D)-2A-mCherry
Plasmid#118292PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acidDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationSerine 306 changed to Aspartic AcidPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DCX(S306A)-2A-mCherry
Plasmid#118291PurposeExpresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for alanineDepositorInsertDoublecortin (DCX Human)
UseAAVTags2A peptide and mCherryExpressionMammalianMutationchanged Serine 306 to AlaninePromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3DN.Ubc.GFP
Plasmid#24984PurposeDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationY705F--dominant negativePromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBeloBac11loxP2272
Plasmid#60342PurposeThis is a BAC vector that contains both wt and mutant loxP sites.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCEP9 MLH1
Plasmid#16458DepositorInsertMLH1 (MLH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBB572
Plasmid#27397DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI
Plasmid#60421PurposeExpresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI
UseTagsNuclear Export SignalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-enAsCas12f
Plasmid#204642PurposeExpression of enAsCas12f in E. coliDepositorInsertenAsCas12f
UseTags6xHisExpressionBacterialMutationD196K, N199K, G276R, N328G, D364RPromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pV238-vPE
Plasmid#225258PurposeMammalian expression of vPEDepositorInsertvPE
UseCRISPRTagsExpressionMammalianMutationR221K K848A H982A N1317RPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro
Plasmid#213030PurposeExpresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV5cap
Plasmid#198015PurposeFor high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV5 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET/ELP-I-CAT
Plasmid#71461PurposeELP-intein purification systemDepositorTypeEmpty backboneUseTagsELP ((VPGXG)110) and inteinExpressionBacterialMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 eta
Plasmid#116887PurposeExpresses HA-tagged human 14-3-3 eta in mammalian cellsDepositorInsert14-3-3 eta (YWHAH Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin E
Plasmid#8963DepositorInsertcyclin E (CCNE1 Human)
UseTagsGPI linkerExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-kanMX6
Plasmid#39294DepositorInsertKanR
UseYeast genomic targetingTags13Myc, A. gossypii translation elongation factor …ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS2-TVA-eGFP-N2cG
Plasmid#175439PurposeCre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAICS-EGFP
Plasmid#99108PurposeExpresses human PAICS-EGFP fusion in mammalian cellsDepositorInsertPAICS (PAICS Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GRP78/BiP
Plasmid#32701DepositorInsertGRP78/BiP (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-GCaMP6s
Plasmid#192945PurposeTo drive GCaMP6s under the control of mouse oxytocin promoterDepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a-V106W
Plasmid#215824PurposeExpress CBE6a-V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6a V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135Purposecan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiCreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->GFPlig
Plasmid#162223PurposeLentiviral vector for constitutive expression of GFP ligand in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ADRP
Plasmid#87161PurposeFluorescent protein localized to lipid droplets (LDs)DepositorInsertADRP (PLIN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-TM
Plasmid#207320PurposeAllows cloning of protein coding sequences to be incorportated into envelope of virionsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inse…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP1-10
Plasmid#68715PurposeSplit GFP assayDepositorInsertGFP1-10
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
JAK2 (V617F)-pcw107-V5
Plasmid#64610Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertJAK2 (JAK2 Human)
UseLentiviralTagsV5ExpressionMutationV617FPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMS
Plasmid#51543PurposeInducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2DepositorUseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pmCherry-C1-RanQ69L
Plasmid#30309DepositorInsertRanQ69L (RAN N/A)
UseTagsFLAG-CherryExpressionMammalianMutationGlutamine 69 is changed to LeucinePromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5a-7
Plasmid#55126PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab5a
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSE401
Plasmid#62202PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-mTSPAN4-mCherry
Plasmid#210330PurposeOverexpression of mTSPAN4DepositorInsertmTSPAN4 (Tspan4 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only