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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pPICZ-pre-Ost1-pro-alphaf-Btl2
Plasmid#117665PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-pro-alphaf-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-K0XCK7
Plasmid#103118PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HeFm1SpCas9
Plasmid#92110PurposeExpression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)
Plasmid#75471PurposeTranscriptional unit for kanamycin resistance geneDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAgrobacterium tumefaciens Nopaline synthase promo…Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
Plasmid#216248PurposeHDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSDDepositorInsertHDR template for human RANGAP1 (RANGAP1 Human)
UseCRISPR and TALEN; Hdr templateTagsminiIAA7.3xFlag.P2A.BSDExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
IF-GFP-ATRX
Plasmid#45444PurposeATRX expression vectorDepositorInsertATRX (ATRX Human, Mouse)
UseTagsGFP and HAExpressionBacterial and MammalianMutationisoform 2, missing codon E124PromoterPCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
scFv-sfGFP-DNMT3A1
Plasmid#102278PurposeThe plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1DepositorInsertDNMT3A1 (DNMT3A Human)
UseLentiviralTagsscFV-sfGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF
Plasmid#74924PurposeMammalian expression of human NSF full-length (744 aa)DepositorInsertNSF (NSF Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCXLE-hUL + mTAGBFP2
Plasmid#74944PurposeDosage control and tracing of reprogramming episomesDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV.EF1α.mEmerald.CD9.miR9T
Plasmid#170452PurposeLentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.DepositorInsertCD9 (CD9 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterEF1aAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shGFP
Plasmid#110318PurposeControl (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2DepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
Plasmid#216247PurposeExpress OsTIR1.F74A mutant with mCherry tag for AID2DepositorInsertOsTIR1.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#165493PurposeNeuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTagsdarkVenusExpressionMammalianMutationPromoterCaMKIIAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-GFP-LoxP-SNAP-TERT
Plasmid#71391PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
Plasmid#176027Purposeconstruct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometryDepositorInsertsrtTA3
Luc2
mtagBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSHUT4-eYFP
Plasmid#133094PurposeAgrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHIDepositorInsertenhanced yellow flourescent protein
UseFungal expressionTagsExpressionMutationPromoterTEF-1αAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only