1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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1. pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS

   Plasmid

   #154225

   Purpose

   Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   pgndA:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   pGndA (An11g02040 A. niger)

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB6-Lrig1-T2A-tdTomato-FNF-TK

   Plasmid

   #134320

   Purpose

   B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Lrig1 (Lrig1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tdTomato

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB6-Cdk6-T2A-td-sfGFP-FNF-TK

   Plasmid

   #134321

   Purpose

   B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tandem dimer-superfolder…

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ-pre-Ost1-pro-alphaf-Btl2

   Plasmid

   #117665

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-pro-alphaf-Btl2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-K0XCK7

   Plasmid

   #103118

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   K0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)

   Plasmid

   #75471

   Purpose

   Transcriptional unit for kanamycin resistance gene

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   NptII
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Agrobacterium tumefaciens Nopaline synthase promo…

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. IF-GFP-ATRX

   Plasmid

   #45444

   Purpose

   ATRX expression vector

   Depositor

   Michael Dyer

   Article

   Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

   Insert

   ATRX (ATRX Mouse, Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Bacterial and Mammalian

   Mutation

   isoform 2, missing codon E124

   Promoter

   PCMV

   Available since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSH-CAG-AtAFB2.F74A-IRES-puro

    Plasmid

    #216245

    Purpose

    Express AtAFB2.F74A mutant without tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.EF1α.mEmerald.CD9.miR9T

    Plasmid

    #170452

    Purpose

    Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

    Depositor

    Tsuneya Ikezu

    Article

    Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Flag-NSF

    Plasmid

    #74924

    Purpose

    Mammalian expression of human NSF full-length (744 aa)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSYC-176

    Plasmid

    #178961

    Purpose

    Expresses human GFAP fused to 3xFLAG tag in mammalian cells

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Insert

    Glial fibrillary acidic protein (GFAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCXLE-hUL + mTAGBFP2

    Plasmid

    #74944

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    LMyc/Lin28 (MYCL, LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-TRC.mKO2_shGFP

    Plasmid

    #110318

    Purpose

    Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

    Plasmid

    #216247

    Purpose

    Express OsTIR1.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    OsTIR1.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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