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Plasmid#196513PurposeExpress single-chain pMHC with sequencing tag for 10x genomicsDepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84C, 10xgenomics compatible tagPromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FAM134C-GFP
Plasmid#201927PurposeFor lentiviral transduction of FAM134C-GFPDepositorInsertFAM134C (RETREG3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-Nanoluc-ccdB
Plasmid#87078PurposeLentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12D
Plasmid#58902PurposeExpresses human Kras with a G12D MutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12D mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.1
Plasmid#36267DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
cyto-Ruby3-iATPSnFR1.0
Plasmid#102551Purposeexpresses cytoplasmic ATP sensor in mammalian cells with red reference proteinDepositorInsertRuby3-iATPSnFR1.0
UseTagsRuby3ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pol
Plasmid#65520PurposeExpresses HBV polymerase in mammalian cellsDepositorInsertHBV polymerase protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee
Plasmid#140836Purposemammalian expression of SpiCee, a buffer of calcium that prevents downstream signalingDepositorInsertSpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tet3G blasticidin
Plasmid#128061PurposeLentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…TagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-30)
Plasmid#199167Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(-30)
UseTagsMGHHHHHHGGAExpressionMutationT19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II
Plasmid#52092PurposeRetroviral expression of all four WT CD3 subunits, co-expressed with EGFPDepositorInsertsUseRetroviralTagsF2A, IRES-EGFP, P2A, and T2AExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab7a-7
Plasmid#55127PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab7a (RAB7A Human)
UseTagsmCherryExpressionMammalianMutationN117S in NM_004637.5PromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Flag-His-ATM wt
Plasmid#31985PurposeMammalian expression of human ATM with Flag and His tagsDepositorInsertATM wild-type (ATM Human)
UseTagsFlag and HISExpressionMammalianMutation3' UTR of 400 nucl.PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPEgRNA
Plasmid#172716PurposeDelivery of guide RNA for prime editingDepositorInsertgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-TFR1
Plasmid#176490PurposeFor lentiviral transduction of 3FLAG-TFR1DepositorInsertTFR1 (TFRC Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKOR1-mcs
Plasmid#181755PurposeAllele replacement for Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-EcoEnv
Plasmid#15802DepositorInsertMLV env (ecotropic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1a-TetOn3G
Plasmid#104543PurposePiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoterDepositorInsertTet-On 3G
UseTagsExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-P2A-MCS (Neo)
Plasmid#89181PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/BLM
Plasmid#111766PurposeTo express human BLM protein in human cellsDepositorInsertBLM (BLM Human)
UseTagsFlag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-C1
Plasmid#54605PurposeLocalization: C1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneUseTagsmCerulean3ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP4-8
Plasmid#79813PurposeModified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugationDepositorInsertGentamicin resistance gene
UseHelper bhr plasmid for mobilizing other plasmids …TagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADRB2-Tango
Plasmid#66220PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRB2 (ADRB2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCG-Hc∆18-AA-IL-13
Plasmid#86171PurposePlasmid encodes blinded measles virus H glycoprotein displaying IL-13DepositorInsertMeasles virus H glycoprotein fused to IL-13
UseTags6x HisExpressionMammalianMutationY463A, R515APromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-H8A/K47Q
Plasmid#207322PurposeVSV-G mutant with abolished receptor binding, but maintained fusogenic capacityDepositorInsertVSV-G (VSIVgp4 Vesicular Stomatitis Virus)
UseTagsExpressionMammalianMutationH8A/K47QPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMH-Halo tag
Plasmid#154144PurposeGateway compatible mammalian expression plasmid for halo tag fusion proteinsDepositorTypeEmpty backboneUseTagsHalo tagExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RhoA-wt
Plasmid#12959DepositorInsertRhoA (RHOA Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF2240
Plasmid#141984PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA3 (GATA3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-KRAB-dCas9-P2A-mCherry
Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only