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Plasmid#92203PurposeRetroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)DepositorInsertSNCA (SNCA Human)
UseRetroviralTagsYFP(1-155)ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGCC4
Plasmid#37058DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a
Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-OVOL2-HA
Plasmid#184737PurposeDoxycycline-inducible lentiviral expression of OVOL2DepositorInsertovo like zinc finger 2 (OVOL2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet ONAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-ZscHCVP-TEVPZ
Plasmid#116094PurposeExpresses a combination of proteasesDepositorInsertZscHCVP-TEVPZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1951
Plasmid#105868PurposeHigh copy number plasmid containing Hspa1a (synonym Hsp68) MiniPromoter insertDepositorUseTagsExpressionMutationPromoterHsp68 MiniPromoterAvailable sinceFeb. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR CD19-mMegf10 CAR
Plasmid#113013Purposefor production of lentivirus encoding the CD19 CAR-Pmegf10DepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hUL
Plasmid#27080PurposeIntegration-free (episomal) expression of human L-MYC and LIN28DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
OpenCRISPR-1 sgRNA-16nt stem
Plasmid#221566PurposeExpress OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorInsertOpenCRISPR-1 sgRNA 16nt stem loop
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
OpenCRISPR-1 sgRNA-12nt stem
Plasmid#221567PurposeExpress OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.DepositorInsertOpenCRISPR-1 sgRNA 12nt stem loop
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PSMA4_GFP_Cterm
Plasmid#221514PurposeFluorescently tagged Psma4-GFPDepositorInsertPSMA4 (PSMA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK33-V5-TurboID-GW
Plasmid#196282PurposeGateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell linesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26
Plasmid#201685PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 geneDepositorInsertNme2-ABE8e-i1
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26
Plasmid#201686PurposeAll-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 geneDepositorInsertNme2-ABE8e-i1 (V106W)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU1aAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP670-2-T2A-HO1
Plasmid#197250PurposeExpresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegansDepositorInsertwmiRFP670-2-T2A-HO1
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV INS2x mRFP-3MREs (AAV-beta)
Plasmid#206928PurposeBeta cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterINSx2Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-mScarlet3_N1
Plasmid#189767PurposeConstruct for labelling actin filaments in mammalian cells with lifeact-mScarlet3DepositorInsertLifeAct-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40
Plasmid#100854PurposeAAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, and AAV9InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XMyc-EGFP-OMP25
Plasmid#83355PurposeFor tagging mitochondria with Myc epitopes (Control-MITO construct)DepositorInsert3XMyc-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRASV12
Plasmid#20205DepositorInsertNRas (NRAS Human)
UseTransposonTagsExpressionMutationG12VPromoterAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eGFP
Plasmid#118093PurposeFlourescent reporter for NF-kB activityDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorUseTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedTagsExpressionMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
UseTags6x HIS and TEVExpressionBacterialMutationC78I, I92V, K116N, Q134E, R283W, C287N, V293S, G3…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only