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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SEO-CaMKII-TVA-P2A-N2cG

Plasmid

#172363

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA.SFFV.tRFP

Plasmid

#169941

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

Depositor

Benjamin Ebert

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pOPING

Plasmid

#26046

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

SS peptide-LacZ-KHHHHHH

Use

Tags

KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-LACTB-dAPEX2

Plasmid

#117180

Purpose

Plasmid name in publication: pAAV-DIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2F

Plasmid

#15800

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2F (UBE2F Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v2

Plasmid

#110070

Purpose

Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)

Use

Tags

Halo-TEV-Flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_RB1#2

Plasmid

#174151

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pg-ODD-EGFP

Plasmid

#87211

Purpose

HIF-1a's ODD domain fused to EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain (AA 530-652)

Promoter

CMV

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-HA

Plasmid

#79427

Purpose

Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

Plasmid

#194692

Purpose

hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Hygro (656-4)

Plasmid

#17446

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Lentiviral Prep

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

Plasmid

#114369

Purpose

expresses Flpo recombinase-dependent GtACR2

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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KAT5 sgRNA1

Plasmid

#138187

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT5

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT5 (KAT5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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KAT5 sgRNA2

Plasmid

#138188

Purpose

3rd generation lentiviral gRNA plasmid targeting human KAT5

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

KAT5 (KAT5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap2mut

Plasmid

#8339

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap2mut (BIRC3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

H574A

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
His-MBP human SPOP (aa 28-337)

Plasmid

#52294

Purpose

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

SPOP (SPOP Human)

Use

Tags

His-MBP and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(Q67L)-HA

Plasmid

#79425

Purpose

Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 67 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(T27N)-HA

Plasmid

#79426

Purpose

Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 27 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-CKB

Plasmid

#23742

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CKB (CKB Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(T31N)-HA

Plasmid

#79429

Purpose

Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(Q71L)-HA

Plasmid

#79428

Purpose

Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-hPMCA2wb

Plasmid

#47752

Purpose

mammalian expression of mCherry tagged hPMCA2, wb splice variant

Depositor

Emanuel Strehler

Insert

PMCA2w/b (ATP2B2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

wb splice variant

Promoter

CMV

Available since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only