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pVD1 Multiplexing Edit E4 (GB2241)

Plasmid

#160563

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E4)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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tetO-AKT1

Plasmid

#170682

Purpose

doxycycline-inducible overexpression of AKT1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

AKT1 (AKT1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HAND1

Plasmid

#170689

Purpose

doxycycline-inducible overexpression of HAND1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND1 (HAND1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

Plasmid

#160566

Purpose

GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E3-E4-En-1)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-UbC-Blast-2A-STING-mNeonGreen

Plasmid

#227185

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

Depositor

Nir Hacohen

Article

Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-hPGK-Blast-2A-STING-TurboID

Plasmid

#204713

Purpose

Expresses C-terminal TurboID tagged human STING for proximity ligation.

Depositor

Nir Hacohen

Article

Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_314-HRI-V5

Plasmid

#202434

Purpose

Expression of HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

Plasmid

#136684

Purpose

Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 (BECN1 Human)

Use

Tags

Trigger Factor

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-NR2F1

Plasmid

#170698

Purpose

doxycycline-inducible overexpression of NR2F1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

deletion of 36G- please see depositor comments

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgATF4-1

Plasmid

#202455

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BPV00554 pET21GG2-His(6)-BECN(248-450)

Plasmid

#136685

Purpose

Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 ECD/BARA (BECN1 Human)

Use

Tags

Hexa-Histadine

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d-sgCiSF3B1

Plasmid

#202446

Purpose

Expression of a CRISPRi guide targeting SF3B1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

SF3B1 gRNA (SF3B1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-HRI-V5-IRES-BFP

Plasmid

#202435

Purpose

Lentiviral vector expression of HRI and TagBFP

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBS L30 Flag-Apex-Rab7A

Plasmid

#135652

Purpose

Rab7A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Use

Tags

Apex2 and FLag

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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BPV00605 pET21GG2-His(6)-BECN(1-265)

Plasmid

#136686

Purpose

Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 IDR + CC (BECN1 Human)

Use

Tags

Hexa-Histadine

Expression

Bacterial

Mutation

DNA optimized for E. coli expression

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge