Showing: 1781 - 1800 of 13889 results
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Plasmid#176529PurposeNuclease Cpf1 adapted for Aspergillus speciesDepositorInsertFnCpf1
UseCRISPR; Fungal expressionTagsExpressionMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationD156R, R883K, R887APromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRTagsExpressionMutationE174R, R951K, R955APromoterAvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His
Plasmid#195610PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tagDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT825C, C900G, A901C, A903C, T1131G, T1137C, T1227…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationA822G, A823C, A824TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
UseTagsExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Nuc-mTurq2-Apollo-NADP+
Plasmid#193302PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the nucleusDepositorInsertG6PD
UseTagsSV40 NLS and mTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Mito-mTurq2-Apollo-NADP+
Plasmid#193303PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the mitochondriaDepositorInsertG6PD
UseTagsmTurqoise2 and mitochondrial targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
UseTagsExpressionYeastMutationPromoterAOX1 promoterAvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianMutationPromoterCBHAvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNK6031
Plasmid#219739PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCVDepositorInsertCestrum yellow leaf curling virus (CmYLCV) promoter
UseSynthetic BiologyTagsExpressionPlantMutationPromoterCmYLCVAvailabilityAcademic Institutions and Nonprofits only -
pRSET-XS-VWF
Plasmid#64847PurposeExpresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminusDepositorInsertVenus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
UseTags7X His, Cerulean, and VenusExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
NET39 Short pmRFP-N2 (451)
Plasmid#61981Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET39 (PPAPDC3 Human)
UseTagsmRFPExpressionMammalianMutationShort form of NET39PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1781 - 1800 of 13889 results